1/13. Remission of acute myeloblastic leukemia after severe pneumonia treated with high-dose methylprednisolone.We report a case of acute myeloblastic leukemia (AML)-M2 (by French-American-British classification) with t(8;21) (q22:q22) that was complicated with severe pneumonia. The patient tested positive by fluorescence in situ hybridization (FISH) for AML1 splitting and positive by reverse transcriptase polymerase chain reaction (RT-PCR) for chimeric AML1/MTG8 messenger rna (mRNA), which indicated splitting of the MTG8 gene on chromosome 8 (q22) and the AMLI gene on chromosome 22 (q22). High-dose methylprednisolone was administered, and the leukemic cells disappeared without chemotherapy, although dysplastic hematopoietic cells were observed transiently after the first therapy. After the disappearance of leukemic cells, FISH for AML1 splitting was negative, and real-time PCR results for quantitative chimeric AML1/ MTG8 mRNA were less than the detectable level, however, RT-PCR results for AML1/MTG8 mRNA remained positive. These findings suggest that the patient acquired morphological, cytogenetic. and possibly molecular genetic remission by the synergistic effects of severe infection and high-dose methylprednisolone.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/13. Legionella lansingensis sp. nov. isolated from a patient with pneumonia and underlying chronic lymphocytic leukemia.A Legionella-like organism, strain 1677-MI-H, was isolated from the bronchoscopy washings of a patient with pneumonia who had a 2-year history of progressive, chronic lymphocytic leukemia. The growth characteristics, cellular fatty acids, and ubiquinone content of the isolate were consistent with those for Legionella spp. The isolate was serologically distinct in the slide agglutination test with absorbed antisera. dna hybridization studies showed that strain 1677-MI-H (ATCC 49751) represents a new Legionella species which is named Legionella lansingensis.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
3/13. Granulocytic sarcoma presenting as pneumonia in a child with t(8;21) acute myelogenous leukemia: diagnosis by fluorescent in situ hybridization.Granulocytic sarcoma is a soft tissue collection of leukemic cells. The authors describe a 4-year-old boy with M2 acute myelogenous leukemia (AML) who presented with fever, mild nonproductive cough, and hematemesis. Although he was initially diagnosed with nodular pneumonia, rapid resolution of a pulmonary infiltrate following induction chemotherapy was suggestive of a pulmonary granulocytic sarcoma. interphase fluorescent in situ hybridization (FISH) of the lung biopsy specimen for the t(8;21)(q22;q22) translocation confirmed the retrospective diagnosis of a well-differentiated pulmonary granulocytic sarcoma. Pulmonary granulocytic sarcomas may be underrecognized in children with AML; this may delay anti-leukemic therapy and may lead to ineffective therapy if misdiagnosed as pneumonia.- - - - - - - - - - ranking = 5keywords = hybridization (Clic here for more details about this article) |
4/13. Legionella micdadei pneumonia diagnosed by culture isolation and dna-dna hybridization from bronchial lavage fluid.An 80-year-old man was admitted because of dyspnea on effort. We suspected an acute exacerbation of chronic heart failure and idiopathic interstitial pneumonia caused by right-sided pneumonia. A nodular shadow in right upper lobe spread and consolidated into the airspace, and it failed to improve despite administration of meropenem trihydrate, vancomycin hydrochloride and clindamycin. A definitive diagnosis of Legionella micdadei pneumonia was made on the basis of this organism being isolated in culture from bronchial lavage fluid and subsequent identification of Legionella micdadei using dna-dna hybridization. The airspace consolidation gradually improved following treatment with intravenous erythromycin and minocycline hydrochloride.- - - - - - - - - - ranking = 5keywords = hybridization (Clic here for more details about this article) |
5/13. Haunting appearance of bcr/abl fusion gene products in a patient with therapy related leukaemia.A 81-year-old man was diagnosed as multiple myeloma and had received melphalan for 6 years. After that, he developed acute myeloid leukemia (AML) with monosomy 7 and minor bcr/abl transcripts. fluorescence in situ hybridization identified no detectable level of bcr/abl rearrangement. During chemotherapy for AML, minor bcr/abl transcripts disappeared and instead major bcr/abl transcripts emerged. He died of pneumonia 3 months later. At that time, neither minor nor major bcr/abl transcripts were seen. These observations suggest that certain therapy related leukemia may be susceptible to generate very small clones with bcr/abl rearrangements.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
6/13. Legionella sainthelensi serogroup 2 isolated from patients with pneumonia.Three Legionella-like organisms isolated from patients with pneumonia are shown to belong to the species Legionella sainthelensi by dna hybridization studies and to a new serogroup, serogroup 2, by serological studies (ATCC 49322). L. sainthelensi serogroup 2 and L. santicrucis are indistinguishable by slide agglutination, but are separable on the basis of their cell wall fatty acid profiles.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
7/13. Chronic cavitary respiratory papillomatosis.A 19-year-old white man with multiple recurrences of respiratory papillomatosis was admitted for recurrent left lower lobe pneumonia and lung abscesses. He was found to have a single large laryngeal papilloma, widespread bronchial papillomatosis, and large cavitary lesions of the left lower lobe. A lobectomy was performed. The smooth-walled, squamous-lined cavities contained large numbers of papillomas, which were strongly positive for human papillomavirus type 11 by in situ dna hybridization. Findings of evaluation of the patient's humoral and cell-mediated immunity were within normal limits. Cavitation appears to have resulted from bronchial obstruction, postobstructive pneumonia, and liquefactive necrosis. We speculate that squamous metaplasia allowed the continued proliferation of papillomavirus within the cavities.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
8/13. legionella longbeachae pneumonia: report of two cases.legionella longbeachae serogroup 1 was isolated from the respiratory secretions of two patients with community-acquired pneumonia. One patient had a mild infection without evidence of the involvement of other organs and recovered, in spite of inappropriate antibiotic therapy. The other patient was severely-ill on presentation with multisystem failure and died soon after admission to hospital. The organisms were identified by the immunofluorescence technique and by quantitative dna-hybridization studies. The sources of the infection in these patients are unknown as the organism has never been isolated from the SA environment.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
9/13. Detection of HTLV-III rna in lungs of patients with AIDS and pulmonary involvement.A majority of pediatric patients and rare adult patients with the acquired immunodeficiency syndrome (AIDS) develop a chronic respiratory disorder referred to as "lymphocytic interstitial pneumonitis" (lip). Efforts to identify an infectious agent responsible for this process so far have failed. In this study, frozen sections of lungs from patients with AIDS and pulmonary disease were tested by in situ molecular hybridization for the presence of cells infected with human T-cell lymphotropic virus type III (HTLV-III) and expressing viral rna. In the case of an infant with lip, a relatively high frequency (0.1%) of cells in the lung were found to be positive for HTLV-III rna. This number is the lower limit of total cells infected since the in situ hybridization technique as applied in this study depends on expression of HTLV-III genes, and previous evidence indicates that a proportion of cells infected with HTLV-III may not express viral rna. Moreover, this degree of infection of the lung is likely limited to lip, since in ten patients with AIDS and pulmonary diseases other than lip, only 0% to 0.002% of cells in lung were positive for viral rna expression. Thus, HTLV-III may play a direct causal role in the development of lip in infected patients, implicating its involvement in yet another of the diverse clinical diseases associated with this virus.- - - - - - - - - - ranking = 2keywords = hybridization (Clic here for more details about this article) |
10/13. Second serogroup of Legionella hackeliae isolated from a patient with pneumonia.A Legionella-like organism (strain 798-PA-H, ATCC 35999) isolated from a lung aspirate was shown by dna hybridization to belong to the species Legionella hackeliae. growth, gas-liquid chromatographic, and physiologic characteristics were consistent with those of the type strain of L. hackeliae, Lansing 2 (ATCC 35250). However, 798-PA-H was minimally reactive with L. hackeliae direct immunofluorescence assay conjugate or slide agglutination test antiserum. Cross-absorption studies with 798-PA-H and Lansing 2 antisera supported placing 798-PA-H in a second serogroup of L. hackeliae.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
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