1/12. Synchronous oral leiomyosarcoma and squamous cell carcinoma.An unusual case of synchronous squamous cell carcinoma and leiomyosarcoma of the oral cavity is reported in a patient without any identified environmental risk or predisposing factors. The invasive squamous cell carcinoma involved the tongue, whereas the leiomyosarcoma was located in the soft palate. No immunostaining was found for human papillomavirus or Epstein-Barr virus, and in situ hybridization showed negativity for human papillomavirus dna within the tumor cells. Alterations of bcl -2, c-erb -b2 and Rb oncoproteins were not found immunohistochemically. Overexpression of p53 was detected by immunohistochemistry in both tumors, but p53 gene mutations were not found by polymerase chain reaction. Neither loss of heterozygosity of p53 nor microsatellite instability was detected in this patient. The smooth muscle nature of the leiomyosarcoma was confirmed by immunohistochemical methods. To our knowledge, synchronous smooth muscle and epithelial oral tumors have not previously been reported.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/12. carcinosarcoma of the tongue with cyclin d1 gene amplification.carcinosarcoma of the tongue is a rare malignancy and its molecular aspect is unclear. A case of carcinosarcoma of the tongue in a 51-year-old man is presented. A polypoid tumor of the tongue, measuring 12 x 12 x 6 mm, was resected. Histologically, the tumor was composed of a squamous cell carcinoma and a spindle cell sarcomatous component. We previously showed that one cell-cycle regulator, the cyclin d1 gene, was frequently amplified in esophageal carcinosarcoma, which shows the same morphologic features as carcinosarcoma of the tongue. In this case, we examined whether the cyclin d1 gene is amplified in carcinosarcoma of the tongue as well. fluorescence in situ hybridization analysis revealed that the cyclin d1 gene was amplified in both components of carcinosarcoma of the tongue. The cyclin d1 protein was also detected by immunostaining in both components. Our results suggest that the amplification of cyclin d1 gene plays a role in the molecular pathogenesis of carcinosarcoma of the tongue, at least in some cases.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
3/12. MALT lymphoma at the base of the tongue developing without any background of immunodeficiency or autoimmune disease.We report a very rare case of a mucosa-associated lymphoid tissue (MALT) lymphoma of the base of the tongue. A 61-year-old woman was admitted to our hospital for further examination of a 12 mm x 15 mm x 5 mm tongue tumor. Histological examination of the tumor revealed a marked lymphoepithelial lesion. lymphoma cells expressed CD5(-), CD10(-), CD19( ), CD20( ) on the surface of the cells by fluorescence activated cell sorter, and the genotypic analysis of the tumor cells revealed the presence of immunoglobulin heavy chain rearrangement and the absence of BCL-2 gene rearrangement by southern blot hybridization. Furthermore, neither the t(11;18) (q21;q21) translocation nor trisomy 3 was detected in lymphoma cells by fluorescence in situ hybridization method. The tongue tumor was completely resected and no recurrence has been noted in the 13 months to date.- - - - - - - - - - ranking = 2keywords = hybridization (Clic here for more details about this article) |
4/12. Human papillomavirus type 16 dna in oral white sponge nevus.White sponge nevus (WSN) is a benign hereditary lesion of the mucous membranes. dna extracted from a biopsy specimen of oral WSN was assayed for the presence of dna sequences homologous to human papillomavirus (HPV) types 1, 2, 4, 6, 11, 13, 16, and 18 by Southern blot hybridization. Only HPV-16 homologous dna sequences were detected at a copy number of approximately 200 to 250 genome copies per diploid cell. The viral dna sequences did not appear to be integrated into the host cell chromosome. The finding of HPV-16 in an inherited lesion such as WSN indicates that caution must be exercised in ascribing a causal association in relation to the demonstration of HPV in other mucosal disorders.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
5/12. Human papillomavirus-associated dysplastic condyloma of the tongue treated with Mohs micrographic surgery.BACKGROUND: Human papillomavirus (HPV) has been causally associated with benign and malignant tumors of the oral mucosa. It is found in the majority of both oral condylomas and oral squamous cell carcinomas. Treatment for these lesions consists of destructive or traditional surgical excision, respectively. OBJECTIVE: Mohs micrographic surgery provides excellent margin control and the highest cure rate for cutaneous malignancies, especially squamous cell carcinoma. Therefore, it represents an excellent alternative for the treatment of HPV-associated malignant and premalignant lesions of the oral mucosa, where the high rate of metastatic potential is compounded by anatomic complexity and the limited amount of normal tissue that may safely be sacrificed with wide excisions. RESULTS: A 38-year-old white man presented with a dysplastic condyloma of the lateral tongue. High-risk HPV-16 deoxyribonucleic acid (dna) was detected by fluorescent in situ hybridization. Surgical resection of the dysplastic condyloma of the tongue by Mohs micrographic surgery resulted in complete lesion removal, with no evidence of clinical recurrence 8 months after surgery. CONCLUSIONS: Mohs micrographic surgery should be considered as a treatment option for cancerous or precancerous lesions of the oral mucosa, especially when the latter are associated with high-risk HPV.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
6/12. Infantile condylomata of the oral cavity.A child had condylomata acuminata localized to the oral cavity. Main points of interest were this exclusive localization, the extremely high number of papillary lesions, not reported in the literature until now, and the excellent response to interferon and local applications of podophyllin. Histologic, ultrastructural, and in situ molecular hybridization techniques were performed to make a correct diagnosis. Transmission of the etiologic agent and therapeutic approaches are discussed.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
7/12. Recurrent oral condylomata acuminata and hairy leukoplakia: an early sign of myelodysplastic syndrome in an HIV-seronegative patient.Oral hairy leukoplakia (OHL) has been observed in all risk groups seropositive for HIV infection. Recently, this lesion has also been described in HIV-seronegative patients with immunosuppression of iatrogenic origin. We report on a hiv-1 and hiv-2 seronegative, heterosexual man affected by refractory anemia with ringed sideroblasts (myelodysplastic syndrome), who developed recurrent oral condylomata acuminata and OHL as an early clinical manifestation. The diagnosis of OHL was confirmed by identifying Epstein-Barr viral particles by electron microscopy and by in situ dna hybridization. HIV infection was ruled out using polymerase chain reaction and testing for hiv-1 and hiv-2 antibodies.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
8/12. Classical Hodgkin's lymphoma presenting with tongue involvement: a case report and review of the literature.We report an unusual case of mixed cellularity classical Hodgkin's lymphoma with prominent involvement of the base of the tongue at diagnosis. in situ hybridization findings for Epstein-Barr virus were positive. Waldeyer's ring involvement by Hodgkin's lymphoma is uncommon with < 200 cases reported in the English literature and only 5 previous reports of Hodgkin's lymphoma involving the tongue.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
9/12. Oral hairy leukoplakia is not a specific sign of HIV-infection but related to immunosuppression in general.Oral hairy leukoplakia (HL) has been regarded as an early sign of HIV infection, and its clinical importance related to the poor outcome of the patients has been emphasized. Initially, HL was observed exclusively among male homosexuals, but subsequently demonstrated in all risk groups of HIV infection. The patient described in this article suggests that oral HL is not specific for HIV infection per se, but may be associated with immunosuppression also due to other causes. We describe an HIV-seronegative, heterosexual man suffering from an acute myeloblastic leukemia, who developed clinically and histologically typical HL while on cytostatics. biopsy showed areas with characteristic ballooning cells, and hyphae of yeasts were demonstrated with PAS-stain. Using the in situ hybridization technique, Epstein-Barr virus (EBV) dna with high copy numbers was disclosed in the superficial and intermediate cells, whereas human papillomavirus (HPV) dna (types 6, 11, 16, 18) was not present.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
10/12. Oral hairy leukoplakia in an HIV-negative renal transplant recipient.Oral hairy leukoplakia (HL) has been seen exclusively in those infected with HIV or at risk for AIDS. This case report describes an example of HL seen in a renal transplant recipient who was negative for HIV on serology and culture. The diagnosis of HL was confirmed using in situ hybridization for EBV dna.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
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