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1/13. Rabies surveillance in the united states during 2000.

    During 2000, 49 states, the district of columbia, and puerto rico reported 7,364 cases of rabies in nonhuman animals and 5 cases in human beings to the Centers for disease Control and Prevention, an increase of 4.3% from 7,067 cases in nonhuman animals reported in 1999. Ninety-three percent (6,855 cases) were in wild animals, whereas 6.9% (509 cases) were in domestic species (compared wth 91.5% in wild animals and 8.5% in domestic species in 1999). Compared with cases reported in 1999, the number of cases reported in 2000 increased among bats, dogs, foxes, skunks, and sheep/goats and decreased among cats, cattle, horses/mules, raccoons, and swine. The relative contributions of the major groups of animals were as follows: raccoons (37.7%; 2,778 cases), skunks (30.2%; 2,223), bats (16.8%; 1,240), foxes (6.2%; 453), cats (3.4%; 249), dogs (1.6%; 114), and cattle (1.1%; 83). Ten of the 19 states where the raccoon-associated variant of the rabies virus has been enzootic reported increases in the numbers of cases of rabies during 2000. Among those states that have engaged in extensive wildlife rabies control programs, no cases of rabies associated with the epizootic of rabies in raccoons (or in any other terrestrial species) were reported in ohio, compared with 6 cases reported in 1999. No rabies cases associated with the dog/coyote variant (compared with 10 cases in 1999, including 5 in dogs) were reported in texas, and cases associated with the gray fox variant of the virus decreased (58 cases in 2000, including 38 among foxes). Reports of rabid skunks exceeded those of rabid raccoons in massachusetts and rhode island, states with enzootic raccoon rabies, for the fourth consecutive year. Nationally, the number of rabies cases in skunks increased by 7.1% from that reported in 1999. The greatest numerical increase in rabid skunks (550 cases in 2000, compared with 192 in 1999) was reported in texas. The number of cases of rabies reported in bats (1,240) during 2000 increased 25.4% over the number reported during 1999 (989) and represented the greatest contribution (16.8% of the total number of rabid animals) ever recorded for this group of mammals. Cases of rabies reported in cattle (83) and cats (249) decreased by 38.5% and 10.4%, respectively, whereas cases in dogs (114) increased by 2.7% over those reported in 1999. Reported cases of rabies among horses and mules declined 20% from 65 cases in 1999 to 52 cases in 2000. Four indigenously acquired cases of rabies reported in human beings were caused by variants of the rabies virus associated with bats. One case of human rabies acquired outside the united states that resulted from a dog bite was caused by the canine variant of the rabies virus.
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2/13. Evidence for a relationship between Ehlers-Danlos type VII C in humans and bovine dermatosparaxis.

    Ehlers-Danlos (ED) syndrome type VII is characterized by the accumulation of collagen precursors in connective tissues. ED VII A and B are caused by mutations in the genes of alpha 1 and alpha 2 collagen I which result in the disruption of the cleavage site of procollagen I N-proteinase. The existence of ED VII C in humans has been hypothesized on the basis of a disorder in cattle and sheep related to the absence of the enzyme. We now present evidence for the existence of this disease in humans, characterized by skin fragility, altered polymers seen as hieroglyphic pictures with electron microscopy, accumulation of p-N-alpha 1 and p-N-alpha 2 collagen type i in the dermis and absence of processing of the p-N-I polypeptides in fibroblast cultures.
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3/13. anthrax in Wabessa village in the Dessie Zuria district of ethiopia.

    In 2002 an investigation of sudden death in a goat in Wabessa village in the Dessie Zuria district of ethiopia was undertaken using fresh blood brought to the Kombolcha Regional Veterinary Laboratory. The sample was examined using standard bacteriological techniques and animal pathogenicity tests were also performed. The laboratory investigation revealed bacillus anthracis as the cause of sudden death. Information gathered from stockowners in the same village revealed other similar recent cases and deaths, both in animals and humans, with farmers clearly describing the clinical signs and necropsy findings of anthrax. The disease occurs annually in this area in May and June, and in the 2002 outbreak mortality rates of 7.7%, 32.7% and 47.1% were observed in cattle, goats and donkeys, respectively. This study indicates that the community of this particular village neither knows of, nor practises, any of the conventional methods for anthrax control. The cutaneous form of the disease in humans and the environmental contamination associated with the practise of opening cadavers are briefly described and the findings are discussed with reference to the epidemiology of anthrax in both ethiopia and elsewhere. Control strategies are also recommended.
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4/13. Transmission of mycobacterium tuberculosis from human to cattle.

    We describe the first transmission of mycobacterium tuberculosis from human to cattle confirmed by molecular typing of isolates involved in the transmission. IS6110-based restriction fragment length polymorphism analysis showed that the isolates from the cattle and farm worker who suffered from pulmonary tuberculosis 1 year prior to this case were the same strains.
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5/13. Investigations following initial recognition of Crimean-congo haemorrhagic fever in south africa and the diagnosis of 2 further cases.

    Sera from 124 cattle herds were tested, and antibodies to Crimean-congo haemorrhagic fever (CCHF) were found in 93 herds. The prevalence of antibodies was high in the interior of the country, in excess of 90% in some herds, but was less than 4% in cattle along the coast from Cape Town to East london. Only 17 out of 1109 (1,5%) human residents of 55 farms had antibodies to CCHF, while none of 164 veterinary research workers or 98 veterinarians engaged in farm animal practice had them. Specimens from 130 suspected cases of viral haemorrhagic fever were examined and CCHF was diagnosed only in the patient previously reported as the first case of the disease to be recognized in this country. A further 2 cases of CCHF were diagnosed by examining 318 specimens from patients with nonfatal febrile illness. Both patients had contact with livestock. Increasing awareness of the disease will probably lead to an increase in the number of cases diagnosed, but there are no grounds for concluding that the disease is on the increase.
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6/13. Cutaneous form of bovine papular stomatitis in man.

    A cutaneous form of bovine papular stomatitis (BPS) infection was diagnosed in eight persons at the School of veterinary medicine at Auburn University, Auburn, Ala. The initial outbreak occurred in five persons who were involved in the care of a bull that required manual placement of an oral feeding tube. Confirmation of diagnosis was based on clinical findings, cytopathological effects in tissue culture, and isolation of typical paravaccinia virus particles in tissue culture. Transmission studies were performed successfully in three normal calves using tissue culture prepared from human biopsy material. In man, the cutaneous form of BPS infection shows gross lesions similar to the cutaneous form of contagious ecthyma ("orf") or pseudocowpox ("milkers' nodules") infection. Because BPS in cattle occurs most often without evidence of readily observable lesions, unlike contagious ecthyma in sheep or pseudocowpox in cattle, the transmission of BPS to man in the cutaneous form could occur without apparent source. The mild clinical manifestations make the condition relatively minor; however, the occasional case may have more severe lesions.
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7/13. An unusual sequel to imported salmonella zanzibar.

    In August, 1980 a rare serotype S. zanzibar was isolated in the North of scotland from a man home on leave from malaysia, whence he returned in November having been bacteriologically negative 2 months previously. In December however, S. zanzibar was isolated from a bulk milk sample taken at a nearby dairy farm. No illness occurred among milking cows which had been brought inside from pasture in mid-October. Since 1972 a variety of different salmonella serotypes had been identified in cattle, milk and other samples at this farm, with seagulls being implicated as the vector transmitting infection from the sewage of a local town on to farmland and an adjacent loch. Although water from this source has not been used in recent years for drinking by cattle, it is utilized for washing floors within the dairy premises. Since 1979, following an outbreak affecting consumers, all milk produced at the farm has been pasteurized.
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8/13. Rabies surveillance in the united states during 1994.

    In 1994, 48 states, the district of columbia, and puerto rico reported 8,224 cases of rabies in nonhuman animals and 6 cases in human beings to the Centers for disease Control and Prevention. Nearly 93% (7,632 cases) were wild animals, whereas 7% (592 cases) were domestic species. The total number of reported cases decreased 13.4% from that of 1993 (9,498 cases), with most of the decline resulting from 19.2% fewer cases of rabies in raccoons. Two previously described epizootics of rabies involving the raccoon variant of the rabies virus have converged in north carolina, and the resulting region is now continuous from alabama and florida in the South to maine in the North. Epizootics of rabies in foxes in west central texas and in dogs and coyotes in southern texas continue to expand, with this state reporting 144 rabid foxes, 53 rabid dogs, and 77 of the 85 cases in coyotes during 1994. maine and new hampshire reported cases of rabies in foxes (6 and 9, respectively) for the first time in 10 years. Nationally, reported cases of rabies in dogs (153) increased by 17.7%, whereas cases in cattle (111) and cats (267) decreased by 14.6 and 8.3%, respectively. cats continued to be the domestic animal most frequently reported rabid. Twenty-eight states and the district of columbia reported decreases in rabies in animals in 1994, compared with 22 states, the district of columbia, and puerto rico in 1993. hawaii and nebraska were the only states that did not report cases of rabies in 1994.
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9/13. tinea corporis due to trichophyton verrucosum: report of a patient from the San'in District.

    A 70-year-old Japanese housewife, a resident of Shimane Prefecture, developed an erythematous, annular skin lesion on her right forearm. Mycological examinations revealed that it was tinea corporis caused by trichophyton verrucosum (T. verrucosum). This fungus was then shown to have been transmitted to her from young dairy cattle introduced from the Hokkaido District to the ranch of her son. To the best of our knowledge, she is the first reported patient with tinea due to T. verrucosum who was infected in the San'in District.
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10/13. Defective anion transport and marked spherocytosis with membrane instability caused by hereditary total deficiency of red cell band 3 in cattle due to a nonsense mutation.

    We studied bovine subjects that exhibited a moderate uncompensated anemia with hereditary spherocytosis inherited in an autosomal incompletely dominant mode and retarded growth. Based on the results of SDS-PAGE, immunoblotting, and electron microscopic analysis by the freeze fracture method, we show here that the proband red cells lacked the band 3 protein completely. sequence analysis of the proband band 3 cDNA and genomic dna showed a C --> T substitution resulting in a nonsense mutation (CGA --> TGA; Arg --> Stop) at the position corresponding to codon 646 in human red cell band 3 cDNA. The proband red cells were deficient in spectrin, ankyrin, actin, and protein 4.2, resulting in a distorted and disrupted membrane skeletal network with decreased density. Therefore, the proband red cell membranes were extremely unstable and showed the loss of surface area in several distinct ways such as invagination, vesiculation, and extrusion of microvesicles, leading to the formation of spherocytes. Total deficiency of band 3 also resulted in defective Cl-/HCO3- exchange, causing mild acidosis with decreases in the HCO3- concentration and total CO2 in the proband blood. Our results demonstrate that band 3 indeed contributes to red cell membrane stability, CO2 transport, and acid-base homeostasis, but is not always essential to the survival of this mammal.
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