1/20. Solitary squamous cell papilloma of the lung in a 40-year-old woman with recurrent laryngeal papillomatosis.A rare case of recurrent respiratory papillomatosis (RRP) is reported with a review of the literature. A 40-year-old Japanese woman had suffered from RRP since 1 year of age. She developed a pulmonary squamous papilloma with a thin-walled cavity, which was suspected as being lung carcinoma. The trachea and bronchi around the tumor were intact, and no malignant transformation was present. Two types of human papillomavirus, 6 and 16, were detected, both in the laryngeal and pulmonary papillomas by in situ hybridization and the polymerase chain reaction method. To date, only 40 cases of juvenile laryngeal papilloma with pulmonary involvement have been reported in the English literature.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/20. Squamous papilloma of the esophagus associated with the human papillomavirus.Squamous cell papilloma of the esophagus is a rare lesion involving less than 60 case reports worldwide. These lesions are generally asymptomatic but may at times grow and spread rapidly. One fatality, a result of massive dissemination, has been reported. Until recently, human papillomavirus had not been identified in association with esophageal papillomas. A second case, to the authors' knowledge, of esophageal papillomas associated with human papillomavirus is reported. The virus has been previously shown to be associated with abnormal squamous epithelium in and adjacent to esophageal carcinoma. The virus was identified from biopsy specimens obtained at endoscopy using DNA in situ hybridization techniques. The strain of human papillomavirus identified is similar to those found in the oropharynx and genital tract, raising the possibility of sexual transmission. This case also differs from the previous case report involving the human papillomavirus because of the patient's benign clinical course. Our case serves to highlight differences that are perhaps unique to the human papillomavirus. Multiple papillomas found in a proximal location within the esophagus seem to favor involvement of the human papillomavirus. Isolated lesions located distally appear more characteristic of chronic gastroesophageal reflux as an etiology. The syndrome of squamous cell papillomas involving the esophagus is reviewed in the article.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
3/20. carcinoma cuniculatum of the esophagus.AIMS: Extremely well-differentiated squamous cell carcinoma with the features of so-called carcinoma cuniculatum (CC) is a rare neoplasm. We describe the clinicopathologic findings of the first 2 cases of CC of the esophagus. methods AND RESULTS: Two elderly men presented with symptoms and clinical signs of esophageal malignancy. Repeated endoscopic biopsies of their esophageal tumors were inconclusive. Resection revealed CC of the esophagogastric junction in both cases. The tumors extended into the adventitia but no lymph node metastases were present. in situ hybridization for human papillomavirus HPV subtypes was negative. CONCLUSION: carcinoma cuniculatum is reported for the first time in the esophagus. The diagnosis of this tumor variant is difficult by means of cytological examination or by endoscopic biopsies alone. carcinoma cuniculatum in this location shows biologic features similar to verrucous carcinoma (deep penetration, no lymph nodes metastases, and location at one end of the esophagus). No evidence of human papillomavirus could be demonstrated.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
4/20. Esophageal squamous papillomatosis.Esophageal squamous papillomatosis is rare and has been associated with gastroesophageal reflux and recurrent respiratory papillomatosis. We report a case of extensive esophageal papillomatosis, no airway involvement and a slowly progressive clinical course with progressive strictures and ultimately fatal squamous cell carcinoma. In-situ hybridization performed on biopsy specimens was negative for high-risk human papilloma virus types. Due to the paucity of reported cases, little is conclusively known about the etiology, natural course and best clinical management of this disease. Human papilloma virus has been linked to some, but not all, cases, and the clinical course has been reported to vary from spontaneous regression to malignant transformation. Surveillance for malignancy by conventional endoscopic biopsies or computed tomography scan appears to have low sensitivity. This case illustrates the difficulties in clinical management and establishing a definite etiology in esophageal squamous papillomatosis.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
5/20. Laryngeal carcinoma associated with human papillomavirus type 16.The advent of molecular hybridization technology has provided evidence supporting human papillomavirus as an etiologic agent of laryngeal carcinoma. Using Southern blot analysis, we identified human papillomavirus type 16 DNA associated with an invasive laryngeal carcinoma. The virus genome did not appear to be integrated into the host genome, as is often the case with anogenital tumors. Laryngeal carcinoma usually arises on the true vocal cords of individuals who demonstrate demographic and lifestyle risk factors for this cancer. The patient, an adult male, has no identifiable risk factors for laryngeal carcinoma other than papillomavirus infection. Our finding suggests that the application of hybridization analysis to all cases of laryngeal cancer would promote understanding of the association between human papillomavirus and laryngeal carcinoma.- - - - - - - - - - ranking = 2keywords = hybridization (Clic here for more details about this article) |
6/20. Molecular cloning and characterization of a new subtype of human papillomavirus 6 DNA.A new subtype of human papillomavirus (HPV) 6 was molecularly cloned from a laryngeal papilloma specimen. The DNA of this isolate showed a strong hybridization signal with HPV 6b DNA under stringent conditions. Comparison of restriction enzyme fragment patterns of this HPV DNA with HPV 6b DNA revealed that this DNA is not identical to HPV 6b DNA. Thus, it represents a new subtype of HPV 6 DNA, which we have tentatively designated as HPV 6e DNA. A map of the physical structure of HPV 6e was also established.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
7/20. Laryngeal papillomatosis with human papillomavirus DNA contracted by a laser surgeon.A 44-year-old laser surgeon presented with laryngeal papillomatosis. In situ DNA hybridization of tissue from these tumors revealed human papillomavirus DNA types 6 and 11. Past history revealed that the surgeon had given laser therapy to patients with anogenital condylomas, which are known to harbor the same viral types. These findings suggest that the papillomas in our patient may have been caused by inhaled virus particles present in the laser plume.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
8/20. Human papillomavirus type 11DNA in papillary squamous cell lung carcinoma.We report a case of papillary squamous cell carcinoma of the lung developing in relation to a condylomatous papilloma and related to human papillomavirus (HPV) infection. The viral origin of the bronchial papillomatous lesion is strongly suggested by cytological and histological features with marked condylomatous changes. No viral capsid antigen was detected by immunohistochemistry. Transmission electron microscopy failed to reveal intranuclear viral-like particles in the papillary part of the carcinoma, but typical ultrastructural koilocytotic cells with irregular nucleus and coarse chromatin were observed. HPV DNA type 11 was detected by in situ hybridization using biotinylated probes on paraffin-embedded specimens, under stringent conditions (Tm-12 degrees, 50% formamide). Papillary squamous cell carcinoma may result from the malignant conversion of benign squamous papilloma of the bronchus. HPV type 11 may be associated with malignant conversion of benign papilloma of the pulmonary tract, as in the upper respiratory tract. in situ hybridization with biotinylated probes is a relatively simple and appropriate method for retrospective analysis of HPV DNA sequences in surgical specimens.- - - - - - - - - - ranking = 2keywords = hybridization (Clic here for more details about this article) |
9/20. Human papillomavirus type 11 DNA in squamous cell carcinomas and pre-existing multiple laryngeal papillomas.The case histories of 4 patients are presented, who developed an invasive squamous cell carcinoma from pre-existing multiple laryngeal papillomatosis (two juvenile-onset and two adult-onset) during a long latency period. A series of 14 routinely processed, paraffin-embedded biopsies obtained from these patients (including both benign papillomas and carcinomas) were analysed using in situ DNA-hybridization technique with 35S-labelled Human papillomavirus (HPV) dna probes of HPV types, 6, 11, 16, 18 and 30. The lesions were also assessed by indirect immunoperoxidase (IP-PAP) technique to demonstrate the expression of HPV structural proteins. On light microscopy, morphology was consistent with HPV infection in all 14 biopsies. HPV antigen expression could not be demonstrated in any of the papillomas or carcinomas, however. HPV 11 DNA was present in high copy numbers in both the benign and malignant lesions of 2 patients, both presenting with the juvenile-onset disease. The present findings provide support for the role of HPV as an etiological agent in laryngeal squamous cell carcinoma, most probably acting synergistically with chemical or physical carcinogens (one patient received irradiation and 2 others were smokers). It seems clear that an infection by the 'low risk' HPV types 6 and 11 by no means excludes the possibility of developing a laryngeal malignancy, so far ascribed to the 'high risk' type HPV 16 only.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
10/20. Chronic cavitary respiratory papillomatosis.A 19-year-old white man with multiple recurrences of respiratory papillomatosis was admitted for recurrent left lower lobe pneumonia and lung abscesses. He was found to have a single large laryngeal papilloma, widespread bronchial papillomatosis, and large cavitary lesions of the left lower lobe. A lobectomy was performed. The smooth-walled, squamous-lined cavities contained large numbers of papillomas, which were strongly positive for human papillomavirus type 11 by in situ DNA hybridization. Findings of evaluation of the patient's humoral and cell-mediated immunity were within normal limits. Cavitation appears to have resulted from bronchial obstruction, postobstructive pneumonia, and liquefactive necrosis. We speculate that squamous metaplasia allowed the continued proliferation of papillomavirus within the cavities.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
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