1/15. Solitary squamous cell papilloma of the lung in a 40-year-old woman with recurrent laryngeal papillomatosis.A rare case of recurrent respiratory papillomatosis (RRP) is reported with a review of the literature. A 40-year-old Japanese woman had suffered from RRP since 1 year of age. She developed a pulmonary squamous papilloma with a thin-walled cavity, which was suspected as being lung carcinoma. The trachea and bronchi around the tumor were intact, and no malignant transformation was present. Two types of human papillomavirus, 6 and 16, were detected, both in the laryngeal and pulmonary papillomas by in situ hybridization and the polymerase chain reaction method. To date, only 40 cases of juvenile laryngeal papilloma with pulmonary involvement have been reported in the English literature.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/15. Upper respiratory tract carcinoma with chromosomal translocation 15;19: evidence for a distinct disease entity of young patients with a rapidly fatal course.BACKGROUND: carcinoma of the upper respiratory tract is rare in childhood, and cytogenetic aberrations have not been characterized in this population. The chromosomal translocation 15;19 has been reported four times previously. All patients were young and had tumors arising in the thorax. The three reports that provide clinical follow-up all describe superior vena cava syndrome and death soon after presentation. All tumors were diagnosed as carcinoma (three undifferentiated, one mucoepidermoid), and the authors suggested thymus, lung, or germ cell origin. methods: The authors investigated the clinical and pathologic findings in two patients with poorly differentiated carcinoma showing evidence of t(15;19). This included a 13-year-old girl with a rapidly growing epiglottic mass, leading to superior vena cava syndrome and death and a 12-year-old girl with an aggressive nasopharyngeal mass showing intracranial extension. RESULTS: The laryngeal tumor was poorly differentiated, with vesicular nuclei, prominent nucleoli, extensive necrosis, and a lymphoplasmacytic infiltrate; cells were positive for cytokeratin and negative for lymphoma, melanoma, germ cell, and endocrine markers. Electron microscopy showed rare intermediate junctions and basal lamina. The nasopharyngeal tumor was poorly differentiated with areas of obvious squamous differentiation observed histologically, immunophenotypically, and ultrastructurally. Cytogenetic and fluorescent in situ hybridization studies were consistent with t(15;19)(q13;p13.1) in both cases. Both children received chemo- and radiotherapy. The first child died of disease after 36 weeks; autopsy revealed tumor in the larynx with spread to the skin/subcutis (neck and thorax) and lymph nodes (cervical, subcarinal, and pulmonary hilar). The second child developed widespread bony metastases and died of disease after 13 weeks. CONCLUSIONS: In conjunction with previous reports, the authors' findings show that t(15;19) is part of a distinct clinicopathologic entity characterized by young age, midline carcinoma of the neck or upper thorax, and a rapidly fatal course. female gender and superior vena cava syndrome are common. The histogenesis of these distinctive tumors is unknown. The authors' findings suggest origin in the upper airway, perhaps from submucosal glands.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
3/15. endoreduplication in conjunction with tumor progression in an aneuploid laryngeal squamous cell carcinoma.We report the case of a 58-year-old man who presented with a squamous cell carcinoma pT1a G2 of the left vocal cord. Six months after histologically verified complete resection, the patient experienced an endolaryngeal and extralaryngeal local recurrence pT4 pN2b G2. We applied DNA flow cytometry (FCM) and comparative genomic hybridization (CGH) on both primary and recurrent tumor. The primary tumor and the endolaryngeal compartment of the relapse was an aneuploid cell clone with a FCM DNA index of 1.42 and 1.44, respectively. The extralaryngeal compartment showed a shift featuring a DNA index of 2.78. In the primary tumor and in both compartments of the recurrence there was an identical pattern of complex chromosomal imbalances as detected in CGH (CGH karyotype: rev ish enh [8q24.2-q24.3, 10q26.1-q26.3, 11q24-q25, 12q24.2-q23.33,X], dim [4q, 13q14.3-q31], amp[1p36.1-p36.2]). Hence, the recurrence was not associated with further gains and losses of chromosomal material. However, in the anterior part of the recurrence, the aneuploid tumor cell genome had completely doubled, obviously due to endoreduplication. Immunohistochemical analysis of several cell-cycle regulators revealed altered expression of checkpoint proteins, pointing to a complex disturbance in cell-cycle regulation.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
4/15. Multicolor fluorescence in situ hybridization analysis of a synovial sarcoma of the larynx with a t(X;18)(p11.2;q11.2) and trisomies 2 and 8.Approximately 10% of all mesenchymal malignancies are classified as synovial sarcomas, which show a balanced translocation t(X;18)(p11.2;q11.2) at the cytogenetic level. The occurrence of this neoplasm in the head and neck region is rare, and its lowest frequency is found in the larynx. When synovial sarcomas are present in such unusual locations, diagnosis based solely on histologic features might be problematic, and to our knowledge, cytogenetic data have been reported so far in only one case of synovial sarcoma of the larynx. Because of the rarity and shortage of consistent prognostic markers, there is no clear consensus for the treatment of these patients. cytogenetic analysis of a primary case of synovial sarcoma of the larynx was performed by using a 48-color fluorescence in situ hybridization technique that allows differential staining of short and long chromosome arms to establish the karyotype. We report here the molecular cytogenetic analyses of a synovial sarcoma of the larynx harboring the diagnostic t(X;18), as well as trisomies 2 and 8. The karyotypic information on synovial sarcomas of the larynx is scarce, and our data might add to the diagnosis and prognosis of this tumor.- - - - - - - - - - ranking = 5keywords = hybridization (Clic here for more details about this article) |
5/15. Laryngeal carcinoma associated with human papillomavirus type 16.The advent of molecular hybridization technology has provided evidence supporting human papillomavirus as an etiologic agent of laryngeal carcinoma. Using Southern blot analysis, we identified human papillomavirus type 16 DNA associated with an invasive laryngeal carcinoma. The virus genome did not appear to be integrated into the host genome, as is often the case with anogenital tumors. Laryngeal carcinoma usually arises on the true vocal cords of individuals who demonstrate demographic and lifestyle risk factors for this cancer. The patient, an adult male, has no identifiable risk factors for laryngeal carcinoma other than papillomavirus infection. Our finding suggests that the application of hybridization analysis to all cases of laryngeal cancer would promote understanding of the association between human papillomavirus and laryngeal carcinoma.- - - - - - - - - - ranking = 2keywords = hybridization (Clic here for more details about this article) |
6/15. Molecular cloning and characterization of a new subtype of human papillomavirus 6 DNA.A new subtype of human papillomavirus (HPV) 6 was molecularly cloned from a laryngeal papilloma specimen. The DNA of this isolate showed a strong hybridization signal with HPV 6b DNA under stringent conditions. Comparison of restriction enzyme fragment patterns of this HPV DNA with HPV 6b DNA revealed that this DNA is not identical to HPV 6b DNA. Thus, it represents a new subtype of HPV 6 DNA, which we have tentatively designated as HPV 6e DNA. A map of the physical structure of HPV 6e was also established.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
7/15. Laryngeal papillomatosis with human papillomavirus DNA contracted by a laser surgeon.A 44-year-old laser surgeon presented with laryngeal papillomatosis. In situ DNA hybridization of tissue from these tumors revealed human papillomavirus DNA types 6 and 11. Past history revealed that the surgeon had given laser therapy to patients with anogenital condylomas, which are known to harbor the same viral types. These findings suggest that the papillomas in our patient may have been caused by inhaled virus particles present in the laser plume.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
8/15. Human papillomavirus type 11 DNA in squamous cell carcinomas and pre-existing multiple laryngeal papillomas.The case histories of 4 patients are presented, who developed an invasive squamous cell carcinoma from pre-existing multiple laryngeal papillomatosis (two juvenile-onset and two adult-onset) during a long latency period. A series of 14 routinely processed, paraffin-embedded biopsies obtained from these patients (including both benign papillomas and carcinomas) were analysed using in situ DNA-hybridization technique with 35S-labelled Human papillomavirus (HPV) dna probes of HPV types, 6, 11, 16, 18 and 30. The lesions were also assessed by indirect immunoperoxidase (IP-PAP) technique to demonstrate the expression of HPV structural proteins. On light microscopy, morphology was consistent with HPV infection in all 14 biopsies. HPV antigen expression could not be demonstrated in any of the papillomas or carcinomas, however. HPV 11 DNA was present in high copy numbers in both the benign and malignant lesions of 2 patients, both presenting with the juvenile-onset disease. The present findings provide support for the role of HPV as an etiological agent in laryngeal squamous cell carcinoma, most probably acting synergistically with chemical or physical carcinogens (one patient received irradiation and 2 others were smokers). It seems clear that an infection by the 'low risk' HPV types 6 and 11 by no means excludes the possibility of developing a laryngeal malignancy, so far ascribed to the 'high risk' type HPV 16 only.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
9/15. Chronic cavitary respiratory papillomatosis.A 19-year-old white man with multiple recurrences of respiratory papillomatosis was admitted for recurrent left lower lobe pneumonia and lung abscesses. He was found to have a single large laryngeal papilloma, widespread bronchial papillomatosis, and large cavitary lesions of the left lower lobe. A lobectomy was performed. The smooth-walled, squamous-lined cavities contained large numbers of papillomas, which were strongly positive for human papillomavirus type 11 by in situ DNA hybridization. Findings of evaluation of the patient's humoral and cell-mediated immunity were within normal limits. Cavitation appears to have resulted from bronchial obstruction, postobstructive pneumonia, and liquefactive necrosis. We speculate that squamous metaplasia allowed the continued proliferation of papillomavirus within the cavities.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
10/15. Changes in human papillomavirus typing of recurrent respiratory papillomatosis progressing to malignant neoplasm.A patient with recurrent respiratory papillomatosis progressed spontaneously to carcinoma resulting in death. Serial biopsy specimens obtained during a 9-year course of this patient's disease were tested via polymerase-chain reaction and Southern hybridization to detect the human papillomavirus (HPV) types present during the patient's course of disease. Early biopsy specimens were positive for HPV types 6 and 11. As atypia progressed to carcinoma, HPV types 6 and 11 remained present, but the signal decreased and HPV 16 became increasingly positive. The significance of these findings will be discussed.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
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