1/3. aeromonas jandaei and aeromonas veronii dual infection of a human wound following aquatic exposure.Exudate removed from an infection that developed below the left eye of a 10-year-old male following a previously inflicted wound after aquatic exposure was cultured and revealed two different aeromonas spp. Further characterization showed that one strain was phenotypically identical to aeromonas veronii, while the other strain was confirmed by dna hybridization analysis to be aeromonas jandaei sp. nov. This is the first report of these more recently described aeromonads, thus far rarely reported from clinical disease, occurring simultaneously in a human infection.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/3. xenorhabdus luminescens (dna hybridization group 5) from human clinical specimens.An unusual isolate from a human leg wound was identified as xenorhabdus luminescens. This finding led to the discovery or isolation of four additional strains, two from blood and two from wounds. Three of the five strains were from patients in San Antonio, Tex. Three strains were studied by dna-dna hybridization (S1 nuclease-trichloroacetic acid method) and were 77 to 100% related to each other, 34% related to the type strain of X. luminescens, 35 to 40% related to three of Grimont's other dna hybridization groups of X. luminescens, and 9% related to the type strain of xenorhabdus nematophilus. The new group of five strains was designated X. luminescens dna hybridization group 5. All five strains were very inactive biochemically and fermented only D-glucose and D-mannose. The key reactions for recognizing this new organism are yellow pigment production, negative test for nitrate reduction to nitrite, weak bioluminescence (10 to 15 min of dark adaptation is required to see the weak light produced), and a unique hemolytic reaction on sheep blood agar plates incubated at 25 degrees C. Two case histories of strains from wounds are given; these suggest that X. luminescens dna hybridization group 5 may be a new bacterial agent that causes wound infections. The two cases of wound infection, along with the two blood isolates, suggest that the new organism is clinically significant.- - - - - - - - - - ranking = 8keywords = hybridization (Clic here for more details about this article) |
3/3. Isolation of gram-positive rods that resemble but are clearly distinct from actinomyces pyogenes from mixed wound infections.Beginning in 1990, gram-positive rods resembling actinomyces pyogenes were found with increasing frequency in mixed cultures from various infectious processes, most of them from patients with otitis, empyema, pilonidal cysts, perianal abscesses, and decubitus ulcers. ribotyping and hybridization showed that these gram-positive rods could be divided into five groups not related to known actinomyces species. Biochemical markers for reliable differentiation into these groups, however, could not be found. Therefore, naming new species is not warranted unless parameters are discovered that allow identification without dna hybridization. These gram-positive rods have been isolated only in mixed cultures with anaerobes, staphylococcus aureus, streptococcus "milleri," enterococci, and gram-negative rods. Their exact role in these possibly synergistic infections needs further investigation.- - - - - - - - - - ranking = 2keywords = hybridization (Clic here for more details about this article) |