1/7. Demonstration of human herpesvirus 8 in a case of primary vaginal epithelioid angiosarcoma by in situ hybridization, electron microscopy, and polymerase chain reaction.We demonstrate the presence of human herpesvirsus 8 (HHV-8) in a primary vaginal location of angiosarcoma (AS) by polymerase chain reaction (PCR), in situ hybridization, and ultrastructural direct visualization of viral particles. The latter two techniques for the first time confirm HHV-8 detection in an AS by PCR; these results contribute to the debate caused by the controversial data produced by the almost exclusive use of PCR for investigating the possible presence of HHV-8 in AS, and its possible implications. Moreover, the investigated AS is the seventh published primary vaginal one, and the fourth unrelated to radiotherapy. Interestingly, the affected patient had used a ring pessary for 10 years because of an uterovaginal prolapse.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/7. Multiple HPV 16-related squamous cell carcinomas of the vulva, vagina, anus, skin and cervix in a 31-year-old woman.A 31-year-old woman is reported with in the genital region multiple squamous carcinomas of the skin (buttock), vulva, vagina, anus and cervix uteri. All these carcinomas were HPV 16 positive as tested by DNA in situ hybridization. The existence of areas with normal epithelium between all tumor localisations and the absence of distant metastases indicate multicentric development of these multiple carcinomas. The presence of HPV 16 DNA in all carcinoma cells, as detected by DNA in situ hybridisation, argues for an etiological role of HPV 16 in the development of these multiple tumors.- - - - - - - - - - ranking = 0.2keywords = hybridization (Clic here for more details about this article) |
3/7. Longitudinal study of human papillomavirus infection of the female urogenital tract by in situ hybridization.The recurrence and/or persistence of human papillomavirus (HPV)-associated lesions constitute a well-recognized clinical problem; yet, few studies have demonstrated and typed the HPV within these recurrence lesions. A patient with a 17-year history of anogenital condylomata presented with an unusual bladder tumor that had histologic features of HPV infection. This prompted a longitudinal analysis of HPV DNA in archival material from these urogenital lesions. Colorimetric in situ hybridization was used to assay for HPV types 6, 11, 16, and 18. Human papillomavirus types 6 and 11 were present throughout the period of follow-up in all the condylomatous lesions, including those identified in the bladder, suggesting a common source of infection and spread. By contrast, HPV type 16 was associated only with the vaginal intraepithelial neoplasia that was identified midway through the period of follow-up, and it was apparently cured by excision of the lesion.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
4/7. Vaginal condylomata acuminata after McIndoe neovagina creation.The author encountered vaginal condylomata acuminata in two women after McIndoe neovagina creations. By in situ DNA hybridization, the authors identified human papillomavirus type 6 in both women. The large exophytic pattern of growth in the neovagina was more characteristic of that encountered on the natural keratinized squamous epithelium of the vulva and perianal area than of the micropapillary and small flat lesions usually found on natural vaginal mucosa. These observations suggest that (1) this is not a rare infection, considering the low frequency of McIndoe neovagina creations; (2) the development of genital warts is probably related to contact with the etiologic virus; and (3) the intrinsic nature of the infected epithelium, as well as the new "vaginal" environment, may help determine the gross characteristics of the lesions. women with neovaginas should be encouraged to have their sex partners use condoms, despite the lack of a need for contraception.- - - - - - - - - - ranking = 0.2keywords = hybridization (Clic here for more details about this article) |
5/7. female genital tumors associated with human papillomavirus infection, and the concept of genital neoplasm-papilloma syndrome (GENPS).With the recent development of new analytic methods, notably the DNA hybridization technique, many benign and malignant gynecologic tumors including carcinoma in situ, verrucous carcinoma and some invasive carcinomas of the vulva, the vagina and the cervix are found to be associated with human papillomavirus infection. Benign warts and multiple neoplasms frequently appear synchronously or metachronously in a single patient, and thus present as the genital neoplasm-papilloma syndrome (GENPS). Various methods of human papillomavirus identification and a spectrum of benign and malignant female genital tumors proven to contain human papillomavirus are reviewed and summarized.- - - - - - - - - - ranking = 0.2keywords = hybridization (Clic here for more details about this article) |
6/7. Presence of human papillomavirus in verrucous carcinoma (Ackerman) of the vagina. Immunocytochemical, ultrastructural, and DNA hybridization studies.Human papillomavirus (HPV) genomes were identified in two cases of verrucous carcinoma of the vagina, using Southern blot DNA hybridization under low-stringency conditions. Type (group) 6 HPV DNA (HPV-6) was identified, using molecularly cloned HPV-1 through HPV-6 dna probes under high-stringency conditions in both cases. In addition, DNA extract in one case hybridized with HPV-1, HPV-3, and HPV-4 dna probes. No HPV structural proteins were demonstrated in either case by immunocytochemical tests, using HPV antibodies. In one case viruslike intranuclear particles were observed by transmission electron microscopy. These two cases suggest a strong associative relationship between HPV and verrucous carcinoma (Ackerman) of the lower part of the genital tract.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
7/7. Specific rearrangements of human papillomavirus DNA provide molecular evidence for genetic heterogeneity of primary cervical cancers, recurrencies, and lymph node metastases in two patients.Two different characteristic patterns of human papilloma virus (HPV) type 16 DNA were found by Southern blot hybridization in four pelvic and paraaortic lymph node metastases in a patient with FIGO stage IIIb cervical cancer. Both patterns added up to give the HPV 16 DNA pattern of the primary tumor. This strongly suggests that the tumor was composed of two distinct compartments, each spawning its own lymph node metastases. A second patient presented with a vaginal tumor 4 years after stage IIb cervical cancer had been treated with hysterectomy only. The vaginal tumor was removed and pelvic lymphadenectomy performed. Integrated HPV 16 DNA was found in the vaginal tumor whereas one involved and one free lymph node contained episomal HPV 16 DNA with a characteristic deletion. The apparent heterogeneity of the cancer cell population may indicate that the metastasis is not related to the vaginal tumor but that it is a late sequel of the cervical cancer. Alternatively the metastasis could have originated from an unsampled portion of the vaginal tumor.- - - - - - - - - - ranking = 0.2keywords = hybridization (Clic here for more details about this article) |