1/8. Penile hybrid verrucous-squamous carcinoma associated with a superficial inguinal lymph node metastasis.A 62-year-old Japanese man who presented with penile carcinoma is reported. The initial exophytic neoplasm excised from the coronal sulcus and prepuce on the abdominal side of the penis was diagnosed histologically as verrucous carcinoma. Twenty-six months after the primary operation, an epithelial neoplasm recurred within the scar of the primary operation. The neoplasm histologically showed verrucous carcinoma and multiple invasive foci of conventional squamous cell carcinoma in the advancing edge of the tumor, as such representing a hybrid verrucous-squamous carcinoma. A lymph node metastasis in the left superficial inguinal lymph node occurred 4 months after the second operation. A total bilateral inguinal lymphadenectomy was performed and revealed no other lymph node metastases. The patient is alive without local recurrence or evidence of metastases in pelvic lymph nodes or visceral organs 2 years after the resection of the hybrid verrucous-squamous carcinoma. The initial verrucous carcinoma, recurrent hybrid verrucous-squamous carcinoma, and metastatic lymph node were negative for human papillomavirus DNA type 6, 11, 16, 18, and 33 sequences by dot blot hybridization of polymerase chain reaction products. The characteristics of hybrid verrucous-squamous carcinoma and importance of lymph node metastasis in penile carcinoma are discussed.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/8. Penile cancer and human papilloma virus (HPV) in a human immunodeficiency virus (hiv)-infected patient.Only a handful of cases of penile carcinoma among human immunodeficiency virus (hiv)-infected males have been reported. This is surprising insofar as other anogenital malignancies associated with human papillomavirus (HPV) are over-represented in hiv-infected men and women. Herein we describe the case of an hiv seropositive 64-year-old Caucasian with a CD4 T-lymphocyte count of 550 cells/mm3 and an invasive squamous cell cancer of the penis. He underwent radical penectomy to treat cancer initially confined to the penile shaft and glans penis, but ultimately succumbed to complications associated with metastatic disease. HPV type 18 was identified by in situ hybridization and polymerase chain reaction (PCR) studies in the primary tumor and in groin and lung metastasis. We also briefly review current thoughts regarding the epidemiology and pathogenesis of penile cancer, particularly in the setting of HPV and hiv co-infection.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
3/8. Cutaneous angiocentric T-cell lymphoma associated with Epstein-Barr virus.BACKGROUND: Two unusual cases of cutaneous angiocentric T-cell lymphoma were found to be associated with Epstein-Barr virus infection. OBJECTIVE: The objective was to study the clinical course and the response of the disease to conventional chemotherapy. methods: Histologic specimens from both patients were studied. Clonal proliferation was assessed by Southern blot hybridization. RESULTS: The disease in both patients was rapidly progressive and responded poorly to aggressive treatment. biopsy specimens showed infiltration of atypical lymphoid cells with angiocentricity and angiodestruction, which probably resulted in the observed tissue necrosis. Clonal proliferation of Epstein-Barr virus DNA was detected in tissue from primary skin lesions and disseminated nasal lesions. CONCLUSION: Epstein-Barr virus-associated angiocentric T-cell lymphoma in our patients was characterized by an aggressive course and resistance to conventional chemotherapy. A search for Epstein-Barr virus and the human T-lymphotropic virus should be performed in patients with atypical features of cutaneous T-cell lymphoma.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
4/8. Verrucous carcinoma of the penis: importance of human papillomavirus typing for diagnosis and therapeutic decision.One case of penile verrucous carcinoma (buschke-lowenstein tumor) undergoing anaplastic transformation and containing human papillomavirus type 6 is presented. The viral genome is detected by in situ hybridization using biotin-labeled cDNA probes. The clinical, histological and virologic criteria of verrucous carcinoma are discussed in comparison to giant condyloma and highly differentiated squamous cell carcinoma. The importance of viral typing determination for further diagnostic and therapeutic procedures is emphasized.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
5/8. Sexually transmitted papilloma viral infection in the male. VII. Is cancer of penis sexually transmitted?Four cases are reported of squamous cell carcinoma of the genital tract in males. The close association of HPV 16/18 with in situ squamous carcinoma affecting the penis is demonstrated. The first documented case of a primary penile squamous cell carcinoma with metastatic deposits yielding positive HPV 16/18 isolates as shown by in situ DNA hybridization again demonstrates a close association of this virus to malignancy in humans. The clinical implications of these findings strongly suggest that squamous cell carcinoma of the penis is a sexually transmitted disease. A thorough examination of both sexual partners is necessary when Bowenoid papulosis or penile carcinoma is present, since the female partner is at high risk for cervical neoplasia.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
6/8. Penile verrucous carcinoma with anaplastic transformation following radiotherapy. A case report with human papillomavirus typing and flow cytometric DNA studies.We report a case of penile verrucous carcinoma with anaplastic transformation after radiotherapy. A 49-year-old man with penile verrucous carcinoma initially underwent excision and chemotherapy followed by radiotherapy when the carcinoma recurred locally. Twenty-four months after the completion of radiotherapy, the verrucous cancer transformed to an anaplastic spindle-cell carcinoma, which resulted in his death 10 months after diagnosis. autopsy confirmed a huge hemorrhagic recurrent lesion and lung metastases. in situ hybridization with biotinylated dna probes did not detect any type of human papillomavirus (HPV) (types 6/11, 16/18, and 31/33/51) in either the primary or the anaplastic tumor. Immunohistochemical HPV capsid antigens were also negative in both lesions. A flow cytometric analysis, using paraffin-embedded materials, showed a diploid DNA content in the excised verrucous carcinoma and an aneuploid content in the anaplastic tumor. This patient most likely had radiation-induced transformation of an anaplastic carcinoma. Wide excision and, if necessary, amputation, are preferred to radiotherapy.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
7/8. A case of verrucous carcinoma associated with human papillomavirus type 16 DNA.We report here a case of verrucous carcinoma which occurred on the penis of a 75-year-old male. The nodule was first noted six months earlier and was whitish, cauliflower-like, and 17 x 19 mm in size. The histopathological examination revealed hypertrophic epidermal proliferation with pale staining keratinocytes, extending into the deep dermis. Partial penectomy and inguinal lymph node dissection were done. No lymph node metastasis was recognized. DNA was isolated from the frozen tumor tissue and examined for the presence of human papillomavirus (HPV) 16, 18, and 33 DNA by the polymerase chain reaction (PCR), using common and specific primers. A 140 base pair (bp) band was amplified and finally determined to be the HPV16 sequence by dot-blot hybridization.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
8/8. HPV-16-positive anal and penile carcinomas in a young man--anogenital 'field effect' in the immunosuppressed male?This report presents the unusual occurrence of metachronous perianal and penile carcinomas in a young, immunosuppressed man. Both anogenital cancers were HPV-16-DNA-positive by the polymerase chain reaction. DNA in situ hybridization analysis of the penile carcinoma revealed HPV-16 in most neoplastic cells. HPV-16 appears to have played a central role in both anogenital cancers in this patient, suggesting that, like in immunologically susceptible women, a carcinogenic 'field effect' may exist in the anogenital area of the immunosuppressed male.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |