1/4. A human breast adenocarcinoma with chromosome and isoenzyme markers similar to those of the HeLa line.pleural effusion was obtained from a 51-year-old black woman who had breast adenocarcinoma and had received chemotherapy and radiation therapy after a radical mastectomy. Cytogenetic and isoenzymic analyses of the cells were performed within a few hours of obtaining the sample. Similar analyses were also done with a cell line established from this effusion. The stemline chromosome number was 35, one of the lowest in human neoplasms. In addition to a marker chromosome involving 1q, which is common in human breast tumors, we found several other marker chromosomes whose G-banding patterns were similar to some of the typical HeLa markers. Genetic signature analysis of 15 isoenzyme loci revealed that 13 were identical to those of HeLa. Both HeLa and the cell line described here express glucose-6-phosphate dehydrogenase type a, yet they were derived from heterozygotic individuals (ab). Our data indicate the necessity to extensive cytogenetic and biochemical analysis before conclusions are made that cell lines are actually intercell-line contaminants.- - - - - - - - - - ranking = 1keywords = nature (Clic here for more details about this article) |
2/4. Human neuroblastoma serially transplanted in nude mice and metastases.A human neuroblastoma from a female patient was directly transplanted into nude mice and serial transplantation was established. histology of the transplanted tumor was almost the same as that of the patient. During serial transplantation, a high frequency of metastasis to distant organs such as ovaries, lymph node and cerebrum was observed, especially in the ovaries where the metastatic rate reached to 66%. Regarding the distribution of organs involved, there is close similarity between that in the patient with neuroblastoma and tumor-bearing mice. This transplantable human neuroblastoma provided a unique research tool for studies on its morphological and biological nature, including metastasis.- - - - - - - - - - ranking = 1keywords = nature (Clic here for more details about this article) |
3/4. Two neoplastic cell lines with unique features derived from Hodgkin's disease.Two in vitro cell lines (L428, L439) were established from pleural effusions of two patients with Hodgkin's disease. The histological diagnosis was ascertained in both cases by two independent pathologists. The neoplastic nature of the culture cells is indicated by the demonstration of several structural and numerical chromosome abnormalities associated with a monoclonal pattern of marker chromosomes. Heterotransplantation in nude mice was achieved by intracranial inoculation and by subcutaneous transplantation of cultured cells embedded in a plasma clot. EBV-specific antigens (EBNA, VCA) were not detectable in either cell line. Ia-like antigens, receptors for T cells, acid phosphatase and acid esterase were shown to be present in the cultured cells. The L428 and L439 cell line lacked surface- or cytoplasmic Ig, HTLA, receptors for C3b, C3d, IgG-Fc, mouse E or sheep E, and were devoid of lysozyme, peroxidase and chloracetate esterase. These features do not correspond to those of B cells, T cells, myeloid cells, monocytes or macrophages; the morphology and the marker pattern of the culture cells, however, is identical with that of freshly obtained Hodgkin (H)- and Sternberg-Reed (SR)- cells, except for the lack of Clg in the in vitro cells, which is explained by the culture conditions. These findings suggest that the L428 and L439 cell lines are indeed derived from H- and SR-cells and offer the possibility of gaining new information upon the nature of Hodgkin's disease.- - - - - - - - - - ranking = 2keywords = nature (Clic here for more details about this article) |
4/4. The reaction of giant cell tumors of bone to transplantation into athymic nude mice: including observations on osteoinduction in the host bed.Fresh biopsy specimens of three typical human giant cell tumors (GCT) were transplanted into athymic mice. The transplants were encapsulated in a host bed-derived fibrous connective tissue envelope. Multinucleated giant cells and mononucleated tumor cells survived chiefly on the vascularized surfaces of the inside of the envelope. fibroblasts proliferated and filled the interior of the transplants where vascularization was relatively insufficient. Since mitotic figures were absent in multinucleated cells, the question arises whether only the mononuclear stroma cells are neoplastic in nature. Multinucleated cells occasionally showed endocytosed pyknotic cells, and survived transplantation for as long as 35 days. When the original tumor contained islands of osteoid and bone cells, the transplants induced differentiation of mouse host bed connective tissue into normal new bone, including bone marrow.- - - - - - - - - - ranking = 1keywords = nature (Clic here for more details about this article) |