1/31. A normal livebirth after intracytoplasmic sperm injection for globozoospermia without assisted oocyte activation: case report.The successful outcome of intracytoplasmic sperm injection (ICSI) with round-headed spermatozoa (globozoospermia) is reported. A couple with infertility secondary to globozoospermia received ICSI treatment. fertilization, cleavage and pregnancy outcomes were recorded. This couple experienced 40, 10 and 42% fertilization rates after ICSI in their first, second and third cycles respectively. pregnancy did not occur in the first or second cycle but was successfully achieved after the third ICSI cycle. It is concluded that current ICSI procedures may overcome the infertility associated with globozoospermia and result in normal healthy livebirth without assisted oocyte activation.- - - - - - - - - - ranking = 1keywords = cycle (Clic here for more details about this article) |
2/31. Successful birth after intracytoplasmic sperm injection for severe male factor infertility in a woman with poor response to controlled ovarian hyperstimulation.Poor responders to controlled ovarian hyperstimulation (COH) present a clinical challenge for in vitro fertilization (IVF) and embryo transfer. The failure of IVF for the treatment of severe male-factor infertility can now be overcome by intracytoplasmic sperm injection (ICSI). The infertile couple documented in this case report came to our hospital because of bilateral tubal occlusion and severe oligoasthenospermia. After three poor-response cycles to COH, one mature oocyte was retrieved and was fertilized using ICSI. Normal fertilization ensued and one good-quality, eight-celled embryo was transferred into the woman's uterus. A single gestation was confirmed by ultrasound seven weeks after transfer. amniocentesis was performed at 16 weeks and demonstrated a normal male fetus with a karyotype of 46,XY. The patient had a spontaneous, normal, vaginal delivery of a 2,650 g healthy male infant.- - - - - - - - - - ranking = 0.33333333333333keywords = cycle (Clic here for more details about this article) |
3/31. Successful pregnancy with intrauterine insemination using vasal sperm retrieved by electric stimulation.OBJECTIVE: To determine the feasibility of electric stimulation applied to the epididymis or vas deferens to retrieve vasal sperm. DESIGN: Two case reports. SETTING: Assisted reproduction practice in a hospital. PATIENT(S): Two patients with retrograde ejaculation and severe asthenozoospermia. INTERVENTION(S): pulse electric simulation was applied to the epididymis or vas deferens to retrieve sperm via a tube cannulated into the vas deferens. After diluting with culture medium, retrieved sperm were used for IUI. MAIN OUTCOME MEASURE(S): The volume of retrieved vasal fluid and motility parameters of the sperm, the achievement of pregnancy, and outcome of pregnancy. RESULT(S): In patient 1, the volumes of vasal fluids and numbers of sperm were 10-40 microL and 31.4-75.9 x 10(6), respectively, during two cycles. sperm motility was 88.4%--93.2%. His wife became pregnant and was delivered of a healthy infant by IUI during the second cycle. In patient 2, 0.6 x 10(6) sperm with a motility of 70% were retrieved and used for IUI. However, his wife did not become pregnant. CONCLUSION(S): Electric vasal sperm retrieval is a feasible method for collecting sperm from the vas deferens in selected patients with male factor infertility.- - - - - - - - - - ranking = 0.66666666666667keywords = cycle (Clic here for more details about this article) |
4/31. Pregnancies after ICSI using sperm with abnormal head-tail junction from two brothers: case report.We report ICSI pregnancies in two couples with a history of long standing primary infertility in which the sperm of the male partner were either acephalic or had abnormal head-midpiece attachments. The two couples, in which the men are brothers, underwent ICSI. Sperm were analysed by transmission electron microscopy and immunocytochemistry with an anti-MPM2 monoclonal antibody. The first couple underwent two ICSI cycles, each consisting of the injection of two mature oocytes and the transfer of two embryos. A successful pregnancy occurred after the second transfer and led to the birth to a healthy girl. The second couple underwent three ICSI cycles, each consisting of the injection of 18 oocytes and the transfer of two embryos; the last of these led to a triple ongoing pregnancy which included two identical twins. Caesarean section led to the birth of three fetal-growth restricted children. This case report demonstrates that ongoing pregnancies can be achieved in cases of abnormal development of the head-neck attachment. The genetic origin of this syndrome is generally accepted, but the phenotypic heterogeneity observed by light and electron microscopy among published cases suggests that there are a variety of genetic causes of this syndrome.- - - - - - - - - - ranking = 0.66666666666667keywords = cycle (Clic here for more details about this article) |
5/31. Different fertilization rates between immotile testicular spermatozoa and immotile ejaculated spermatozoa for ICSI in men with Kartagener's syndrome: case reports.We report two cases of infertility treatment in couples where males suffered from Kartagener's syndrome (KS) and a total absence of motile sperm in the ejaculate. A total of three ICSI cycles was carried out. In all cycles, viable ejaculated or testicular spermatozoa were selected using the hypo-osmotic swelling (HOS) test. Case 1: In the first ICSI cycle total fertilization failure occurred after using ejaculated spermatozoa. In the following cycle testicular spermatozoa were used for ICSI, resulting in 75% fertilized oocytes and a pregnancy. Case 2: In the same ICSI cycle 50% of the oocytes were injected with ejaculated and 50% with testicular spermatozoa. The fertilization rates were 44 and 56% respectively and high quality embryos were achieved in both groups. One single embryo derived from testicular sperm was transferred with a resulting singleton pregnancy. In conclusion, testicular sperm for ICSI seem to have reliable fertilization capacity in men with KS, while ejaculated sperm, even if tested viable, seem more unpredictable. HOS test for selection of viable sperm for ICSI is recommended when ejaculated as well as testicular sperm are used for ICSI.- - - - - - - - - - ranking = 1.6666666666667keywords = cycle (Clic here for more details about this article) |
6/31. Preliminary evidence on the safety of ICSI with testicular spermatozoa in HCV-infected male: a case report.An infertile couple with a hepatitis c (HCV) rna-positive male came to our attention. We were not able to perform an assisted reproduction technology (art) procedure with ejaculated spermatozoa free of HCV rna using gradient centrifugation and swim up, due to retrograde ejaculation and severe male factor. ICSI with testicular spermatozoa was the most rational therapeutic approach. The couple was informed about the lack of adequate data on the safety of this therapeutic approach. The risks of this procedure were accepted by the couple. Testicular sperm aspiration combined with ICSI (TESA-ICSI) was performed. After a negative result for an HCV rna PCR on the embryos' culture medium, four embryos were transferred to the uterine cavity on the third day. The procedure resulted in an ongoing clinical pregnancy, and HCV antibody determinations performed in the mother at the 12th and 24th week of pregnancy were negative. The pregnancy ended at the 39th week due to endo-uterine death. No malformation or hepatic pathologies were found in the conceptus. A second TESA-ICSI cycle is ongoing. This preliminary evidence suggests that, in HCV sero-positive males, ICSI with testicular spermatozoa may be a safe procedure. However, we need more observations to clarify if art is really able to reduce horizontal and vertical transmission of HCV in sero-discordant couples (only the male infected) in comparison with natural conception.- - - - - - - - - - ranking = 0.33333333333333keywords = cycle (Clic here for more details about this article) |
7/31. Evaluation and treatment of familial globozoospermia in five brothers.OBJECTIVE: To document the pathology of five siblings with complete globozoospermia and to report the effectiveness of repeated intracytoplasmic sperm injection (ICSI) treatment as therapy. DESIGN: Case report. SETTING: A tertiary center for assisted reproduction. patients(S): Five siblings with globozoospermia. INTERVENTION(S): Twenty cycles of repeated ICSI treatment. Detailed light and electron microscopy studies were performed on three of the globozoospermic brothers. MAIN OUTCOME MEASURE(S): Clinical pregnancy and live birth after ICSI treatment. RESULT(S): light and electron microscopy showed that all spermatozoa had round heads and no acrosome. Of 129 metaphase-II oocytes injected, 49 fertilized normally, giving an overall fertilization rate of 38% (range, 0-100%). No pregnancies resulted from 13 cycles in brothers 1-3. Brothers 4 and 5 had three pregnancies after seven ICSI cycles, with one live birth and two first-trimester losses. CONCLUSION(S): Despite variable fertilization rates, pregnancies and a live birth can be achieved after repeated ICSI treatment cycles in globozoospermic siblings. No apparent pattern was manifest that reflected the true pathology or determined the outcome of ICSI treatment.- - - - - - - - - - ranking = 1.3333333333333keywords = cycle (Clic here for more details about this article) |
8/31. High rate of premature chromosome condensation in human oocytes following microinjection with round-headed sperm: case report.A young couple proceeded to three ICSI treatment cycles because of male infertility. The semen samples varied between 10 x 10(6) and 36 x 10(6)/ml, 38 and 51% progressive motility but 0% normal morphology. Different types of sperm heads, mostly round-headed with varying spherical appearance (86%) were presented beside acephalic sperm (pinheads; 12%), both one- or two-tailed and the former also without a tail. Very few sperm (2%) exhibited slightly oval-shaped heads. Electron microscopy revealed the absence of the acrosome combined with disturbance of the chromatin condensation among the round-headed sperm. In all three cycles, the fertilization rate using the round-headed sperm fraction was very low with the best result of 2/18 (11%) two-pronucleate oocytes and one one-pronucleate oocyte obtained in the second ICSI cycle. The three oocytes cleaved and were transferred in the 3-4-cell stage without achieving a pregnancy. Of the 29 unfertilized and prepared oocytes from the last two cycles, 27 were informative and revealed the maternal metaphase II chromosomes in the haploid range and a high rate (85%) of premature chromosome condensation (PCC) of the sperm nucleus with remarkable variation in the degree of condensation. Thus, it appears that nearly all round-headed sperm from this patient were incapable of oocyte activation after ICSI, which could be due to non-release (or absence) of an activating factor. As a consequence, PCC was induced in the sperm nuclei by the chromosome condensing factors which were still active in the oopasm of the arrested oocytes.- - - - - - - - - - ranking = 1.3333333333333keywords = cycle (Clic here for more details about this article) |
9/31. Acral and renal malformations following ICSI.This case reports a delivery of a child with limb and renal malformations following intracytoplasmic sperm injection. A 29-year-old woman and a 39-year-old man with primary infertility underwent IVF-ICSI-ET therapy cycle. The couple delivered an infant with acrorenal syndrome. When assisted reproductive techniques are used, especially ICSI, the risk of genetics abnormalities should be discussed in detail with the couple and sufficient informed consent should be obtained before starting IVF-ET procedures.- - - - - - - - - - ranking = 0.33333333333333keywords = cycle (Clic here for more details about this article) |
10/31. Addison's disease presenting as male infertility.OBJECTIVE: To present a case of primary male infertility with severe oligospermia and hyperpigmented skin as the main presenting clinical signs. DESIGN: Case report. SETTING: University-affiliated teaching hospital. PATIENT(S): A 32-year-old male with severe oligospermia and his 31-year-old female partner with normal cycles. INTERVENTION(S): hydrocortisone, fludrocortisone, methimazole, and eventual IVF. MAIN OUTCOME MEASURE(S): Improved semen analysis, correction of Addison's disease, correction of hyperthyroidism, and art pregnancy. RESULT(S): Severe oligospermia of 5 million per milliliter with 15% motility and ACTH level of 2,800 pg/mL on presentation. endocrinology evaluation revealed cortisol of <0.1 microg/dL, and Cortrosyn test showed no response (Addison's disease). The patient was incidentally found to have a thyroid stimulating hormone level of <0.1 microIU/mL, T4 of 9.5 microg/dL, Free T4 Index calculation of 11.6 microg/dL, and quantitative triiodothyronine of 273 ng/dL on presentation. His Addison's disease was managed with hydrocortisone and fludrocortisone, and his hyperthyroidism, with methimazole. Semen parameters increased to 34 million per milliliter with 45% motility and 5% Kruger morphology 1 year after initial presentation. Hormone parameters normalized. Rather than allowing for more time for natural conception or IUI, the couple decided to proceed with IVF because of insurance coverage before semen parameters normalized. CONCLUSION(S): This is the first reported case of Addison's disease presenting as male infertility with hyperpigmentation of the skin being the only other presenting sign. The underlying etiology of the Addison's in this case remains uncertain, but it is presumed because of autoimmunity. Addison's disease may be associated with hyperthyroidism, and hyperthyroidism may have contributed to the oligospermia. This case highlights why men with abnormal semen parameters should be thoroughly evaluated before proceeding with assisted reproduction.- - - - - - - - - - ranking = 0.33333333333333keywords = cycle (Clic here for more details about this article) |
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