1/9. Bone marrow failure associated with herpesvirus 8 infection in a patient undergoing autologous peripheral blood stem cell transplantation.We describe a fatal case of human herpesvirus 8-associated bone marrow failure in a patient who had received intense treatment for Hodgkin lymphoma and was undergoing bone marrow transplantation. Bone marrow failure was resistant to antiviral treatment and a second infusion of autologous stem cells. Human herpesvirus 8 infection continues to be a major concern in transplant recipients in critical condition.- - - - - - - - - - ranking = 1keywords = herpesvirus (Clic here for more details about this article) |
2/9. Human herpesvirus-6 in human lymphomas: identification of specific sequences in Hodgkin's lymphomas by polymerase chain reaction.In search of a possible involvement of the human herpesvirus type 6 (HHV-6) in human Hodgkin's and non-Hodgkin's lymphomas, we studied the levels of anti-HHV-6 antibodies in the sera of 94 cases by an immunofluorescence assay, as well as the presence of HHV-6 sequences in the affected tissues of 66 cases by polymerase chain reaction, using one set of primer oligonucleotides. Our results showed higher anti-HHV-6 antibody titers in human lymphomas than in normal blood donors, but the difference is statistically significant only when normal donors are compared with Hodgkin's lymphoma cases. HHV-6 sequences were detected in 3 of 25 Hodgkin's lymphomas and 0 of the 41 cases of non-Hodgkin's lymphomas studied. The three cases positive for HHV-6 sequences belong to the nodular sclerosis-lymphocyte depletion histologic subtype and share remarkable similarities in their clinical features. Furthermore, Southern blot analysis of total genomic dna obtained from the neoplastic tissues of two of the three patients showed the same restriction fragment length polymorphism. Our results suggest that: (1) the high level of anti-HHV-6 antibodies in Hodgkin's disease is due to an activation of the immune system not related to the presence of HHV-6 sequences in affected lymph nodes; (2) the presence of HHV-6 sequences in human lymphoid tissues is not a frequent event, rather it is in fact a very rare event in non-Hodgkin's lymphomas, while in Hodgkin's cases it is more frequent than previously reported on the basis of Southern blot analysis; and (3) the presence of HHV-6 sequences in Hodgkin's lymphomas may have a relation with the clinical presentation of the disease.- - - - - - - - - - ranking = 0.83333333333333keywords = herpesvirus (Clic here for more details about this article) |
3/9. Recovery of herpesviruses from cerebrospinal fluid of immunodeficient homosexual men.Over a one-year period the cerebrospinal fluid (CSF) obtained from a series of homosexual men immunocompromised with either Hodgkin's disease or acquired immune deficiency syndrome (AIDS) was cultured to assess the frequency with which infectious viruses could be recovered. Of 58 patients examined, 4 (6.9%) had CSF cultures that showed a cytopathology consistent with a virus infection. All isolates proved to be herpesviruses. cytomegalovirus (CMV) and varicella-zoster virus were isolated from CSF obtained from 2 patients with neurological features consistent with a subacute encephalitis common among AIDS patients. CMV was also recovered from the CSF of an AIDS patient who developed an ascending myelitis of herpesvirus origin. Finally, a CSF sample obtained from an immunodeficient homosexual man who showed no detectable neurological abnormalities consistently yielded herpes simplex virus type 1 in culture. These results suggest that seeding of the CSF with infectious virus is an uncommon event in this patient population. However, our experience should not dissuade attempts to culture viruses from CSF in similar cases. Successful isolations may prove beneficial in the diagnosis of an accompanying neurological illness and facilitate treatment with antiviral therapy when indicated.- - - - - - - - - - ranking = 1keywords = herpesvirus (Clic here for more details about this article) |
4/9. Herpes without vesicles: limited, recurrent genital lesions in an immunodebilitated host.We have reported a case of herpes genitalis in a man with acquired immunodeficiency syndrome (AIDS), who was receiving chemotherapy for Hodgkin's disease. The herpes infection was recurrent but limited, without vesicles or the progressive lesions usually seen in AIDS. Studies excluded known causes, and the unusual character of the infection long obscured and delayed diagnosis. Even atypical, culture-negative penile ulcers in immunodebilitated patients may be herpetic. Retrospective study of our patient's penile biopsy specimen using immunoperoxidase reaction was positive for herpesvirus. The existence of effective treatment mandates vigorous pursuit of the diagnosis.- - - - - - - - - - ranking = 0.16666666666667keywords = herpesvirus (Clic here for more details about this article) |
5/9. Hodgkin's disease with specific bullous lesions.A variety of bullous eruptions have been reported in patients with Hodgkin's disease, among them erythema multiforme, herpesvirus infections, bullous impetigo, prurigo-like papules with vesicles, drug eruptions, bullous pemphigoid, dermatitis herpetiformis, and acquired epidermolysis bullosa. We now describe a patient whose bullous eruption was thought initially to be bullous pemphigoid. However, histopathologic examination of a bulla showed a lymphomatous infiltrate beneath an intraepidermal and subepidermal blister. These pathological findings seem to be unique and we interpret them to be those of bullous Hodgkin's disease.- - - - - - - - - - ranking = 0.16666666666667keywords = herpesvirus (Clic here for more details about this article) |
6/9. Herpesvirus colitis: a new cause of diarrhoea in a patient with Hodgkin's disease.A 57 year old woman developed severe diarrhoea during chemotherapy for Hodgkin's lymphoma. Extensive colonic ulceration was observed at necropsy and electron microscopy showed intranuclear virions consistent with herpesvirus infection. Antibody titres and immunoperoxidase studies indicated that the virus was probably herpes simplex.- - - - - - - - - - ranking = 0.16666666666667keywords = herpesvirus (Clic here for more details about this article) |
7/9. Ogilvie's syndrome from disseminated varicella-zoster infection and infarcted celiac ganglia.We report a patient who had refractory Hodgkin's disease and who received an autologous bone marrow transplantation and 8 months later developed abdominal pain associated with acute colonic dilation. The course of the patient was rapidly fatal due to a lobar pneumonia. autopsy revealed signs of disseminated herpesvirus infection with marked hemorrhagic infarction of celiac sympathetic ganglia. This finding supports the hypothesis that denervation caused by virus reactivation and secondary hemorrhage is a main mechanism of acute colonic pseudoobstruction.- - - - - - - - - - ranking = 0.16666666666667keywords = herpesvirus (Clic here for more details about this article) |
8/9. Three cases of human herpesvirus-6 latent infection: integration of viral genome in peripheral blood mononuclear cell dna.saliva and peripheral blood mononuclear cells from three patients, two with lymphoproliferative disorders and one suffering from multiple sclerosis, were examined for the presence of human herpesvirus-6 (HHV-6) genome by using the polymerase chain reaction and Southern blot analysis. The search for anti-HHV-6 antibodies, carried out in the sera of the same cases by an immunofluorescence assay, was negative in two cases at the lowest dilution used (1:40). These three patients had a high number of HHV-6 specific sequences in uncultured peripheral blood mononuclear cells, which are thought to be a normal site of viral latency although, in healthy individuals, the infected cells are extremely rare. In order to gain some insight into the state of the viral genome in this latent HHV-6 infection, we used pulsed field gel electrophoresis to separate HHV-6 dna directly from HHV-6 (strain GS) infected HSB-2 cells and from the peripheral blood mononuclear cells of these three patients. Our study showed the presence of intact viral genome, of the expected length of 170 kb, persisting as free extrachromosomal element in the HSB-2 cells but not in patients' peripheral blood mononuclear cells. On the other hand, in strong contrast with the results obtained in infected HSB-2 dna, the restriction analysis of the three patients' peripheral blood mononuclear cell dna showed fragments of molecular weight constantly higher than the 170 kb segment, indicating that the viral sequences are linked to high molecular weight cellular dna.(ABSTRACT TRUNCATED AT 250 WORDS)- - - - - - - - - - ranking = 0.83333333333333keywords = herpesvirus (Clic here for more details about this article) |
9/9. The evidence of human herpesvirus 6 infection in the lymph nodes of Hodgkin's disease.Human herpesvirus 6 (HHV-6), the causative agent of exanthem subitum, has been implicated in other diseases. Recently HHV-6-specific sequences have been detected by Southern blot analysis and polymerase chain reaction in the lymph nodes of three patients with Hodgkin's disease. The pathological localization of HHV-6, however, is still unknown. In order to study the pathological role of HHV-6 in Hodgkin's disease, we investigated, by immunohistochemical and molecular methods, two lymph node biopsies taken from a 7-year-old boy with Hodgkin's disease during the course of disease evolution. Although the histopathological findings of the first biopsy differed from those of the second, HHV-6 antigens and sequences could be detected in both lymph nodes by immunohistochemistry and in situ hybridization, respectively. HHV-6 was localized in macrophages, predominantly in lymphoid follicles, but not in reed-sternberg cells. Antibody titres to HHV-6 were consistent with reactivation of latency. Neither cytomegalovirus nor Epstein-Barr virus was present. Our data suggest a role for HHV-6 in the pathogenesis of Hodgkin's disease.- - - - - - - - - - ranking = 0.83333333333333keywords = herpesvirus (Clic here for more details about this article) |