1/6. The presence of HPV types 6/11, 16/18, 31/33/51 in Bowenoid papulosis demonstrated by dna in situ hybridization.Bowenoid papulosis (BP) is an unusual dermatosis with variable clinical presentation: multiple, generally small, round, papules, isolated or confluent, with smooth or papillomatous surface, sometimes with desquamation. The colour is variable from rose, reddish-purple to brown. These papules are localized mostly on the genital mucosa or perigenital skin of young adults. The aetiopathogenesis of the disorder is not well defined, but it may be linked to human papillomavirus (HPV) infection. We report a case of BP with some particular aspects: (1) misdiagnosis of the disease for 2 years; (2) contemporaneous presence of different types of HPV.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/6. Analysis of a giant marker chromosome in a well-differentiated liposarcoma using cytogenetics and fluorescence in situ hybridization.Well-differentiated liposarcomas (LPS) are cytogenetically very complex, characterized by giant marker chromosomes, ring chromosomes, and telomeric associations. We report a case of well-differentiated LPS in which the only cytogenetic anomaly was an additional giant marker. In an attempt to identify the origin of this marker, centromeric probes (chosen on the basis of the morphology of the marker) to chromosomes 1,2,3,4,6,7,8,9,10,11,12,16,17, and X and a shared satellite probe for chromosomes 1,5, and 19, were used with fluorescence in situ hybridization (FISH). This was successful at eliminating certain chromosomes as candidates for centromeric trisomy but could not identify the origin of the marker. This case is unusual in that it does not conform to the typical cytogenetic pattern for well-differentiated LPS and is the first known example with an apparently normal diploid karyotype with only one additional change.- - - - - - - - - - ranking = 1.25keywords = hybridization (Clic here for more details about this article) |
3/6. Myeloid sarcoma of the urinary bladder and epididymis as a primary manifestation of acute myeloid leukemia with inv(16).Myeloid sarcoma (MS) of the lower urinary tract is rare. We describe a 47-year-old man with hematuria, who was subsequently found to have MS involving bladder and epididymis. The neoplasm was composed predominantly of blasts that expressed CD68, CD117, myeloperoxidase, and lysozyme, with occasional immature eosinophils. Although blood and bone marrow examinations showed no morphologic evidence of leukemia, conventional cytogenetic studies of marrow demonstrated inv(16)(p13q22) in 4 of 20 metaphases; fluorescence in situ hybridization of the bladder neoplasm also showed inv(16). Following chemotherapy, the patient has been in complete remission for 32 months. In our literature review, we identified 7 cases of MS involving bladder, only 3 without evidence of an associated myeloid neoplasm in marrow, none with cytogenetic data. A high index of suspicion is required to establish the diagnosis of MS involving bladder. cytogenetic analysis is useful for both demonstrating minimal marrow disease and classifying MS in paraffin-embedded tissue sections.- - - - - - - - - - ranking = 0.25keywords = hybridization (Clic here for more details about this article) |
4/6. Bowenoid papulosis of the genitalia associated with human papillomavirus dna type 16 in an infant with atopic dermatitis.A case of bowenoid papulosis occurred in a 2-year-old male with atopic dermatitis. Clinical and histologic features of the lesions were typical, and human papillomavirus type 16 dna was identified using high-stringency hybridization techniques. Although the lesions had been present for approximately one year prior to examination, they subsequently resolved spontaneously over six months. We postulate that our patient's susceptibility to human papillomavirus may have been related to his severe atopic tendency.- - - - - - - - - - ranking = 0.25keywords = hybridization (Clic here for more details about this article) |
5/6. Genital tract papillomavirus infection in children.Genital tract papillomas in five children were examined for the presence of human papillomavirus (HPV) dna by molecular hybridization. Papillomavirus dna was detected in each sample and was identified as HPV-6 (three cases), HPV-6 or HPV-11 (one case), or HPV-16 (one case). These viruses are the same as are responsible for genital papillomas (condylomata) of adults. The transmission of adult genital tract viruses to children occurs primarily by a venereal route but may occur by a nonvenereal route.- - - - - - - - - - ranking = 0.25keywords = hybridization (Clic here for more details about this article) |
6/6. Buschke-Loewenstein tumour infiltrating pelvic organs.We report a 42-year-old hiv-negative patient with a 12-year history of exceptionally extensive genital warts and coexisting verrucous carcinoma of the anogenital region (Buschke-Loewenstein tumour). Masses of both tumour and viral papillomas infiltrated the external genitalia, perineum and buttocks, pelvic diaphragm and parts of the lesser pelvis, as well as the urethra, prostate and parts of the urinary bladder, necessitating repeated surgical intervention and plastic reconstruction. Adjuvant interferon-alpha therapy was given without any lasting effects. Human papillomavirus type 6 was detected by dna in situ hybridization and Southern blot analysis.- - - - - - - - - - ranking = 0.25keywords = hybridization (Clic here for more details about this article) |