1/4. Solitary plasmacytoma of the mandible in a renal transplant recipient.A solitary plasmacytoma occurring in the mandible of a 15-year-old Korean male 6 years after renal transplantation is reported. The tumour presented as a hyperplastic gingival overgrowth in the right madibular molar area. histology and immunohistochemistry revealed plasmacytoma and monoclonality of the kappa chain and IgG. in situ hybridization for Epstein-Barr virus encoded rna (EBER) showed positive signals in the tumour cells. The tumour regressed after reducing the immunosuppressive agents with concurrent radiotherapy. The patient remains in a stable condition with normal renal functions after 7 years without recurrence. This case confirms that Epstein-Barr virus associated B-lymphoproliferative disorders are still a major complication of immunosuppression.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/4. Cytogenetic and fluorescence in situ hybridization analysis of a basal cell adenocarcinoma of the mandible.Basal cell adenocarcinoma (BCAC) of the salivary glands is rare. Distant metastasis to the mandible from a salivary gland tumor is also considered rare. The cytogenetic finding of a case of metastatic BCAC of the mandible is described. We are unaware of earlier reports regarding cytogenetic findings of BCAC either at the primary site or at a distant metastasis site. An 80-year-old female with primary BCAC of the parotid salivary gland underwent parotidectomy and chemotherapy. One year later, a metastatic lesion in the mandible was found. Tissue specimens from the mandibular lesion were tested by the following pathologic methods: hematoxylin-eosin and immunohistochemistry for CK8/18, CK/903, vimentin, and smooth muscle actin. The characteristic histologic architecture of BCAC found in the mandible was similar to that of the earlier findings of the tumor in the parotid gland. A fresh sample from the mandibular lesion was examined by cytogenetics and fluorescence in situ hybridization (FISH), using centromeric probes for chromosomes 4, 8, 10, 18, and 22. A paraffin-embedded sample of the primary tumor was also examined by FISH. Cytogenetic and FISH analyses of the mandibular metastatic lesion revealed a clone with a pericentric inversion of chromosome 17 and a clone with trisomy 4, respectively. trisomy 4 was also found in the paraffin-embedded samples of the primary parotid tumor.- - - - - - - - - - ranking = 5keywords = hybridization (Clic here for more details about this article) |
3/4. Detection of human papillomavirus dna in an ameloblastoma using the in situ hybridization technique.HPV type 18 dna was identified in an intrabony ameloblastoma using radiolabelled in situ hybridization. The viral dna was found in a verrucous lesion in a cystic area of the tumor. The absence of HPV dna in other epithelial areas of the ameloblastoma is suggestive of a secondary infection. HPV is not considered to be an etiological factor in the pathogenesis of this ameloblastoma.- - - - - - - - - - ranking = 5keywords = hybridization (Clic here for more details about this article) |
4/4. Intraoral presentation of anaplastic large-cell Ki-1 lymphoma in association with hiv infection.persons infected with human immunodeficiency virus have an increased risk for development of high-grade, non-Hodgkin's lymphomas. Anaplastic large-cell Ki-1 lymphoma is a recently described lymphoid neoplasm characterized by cellular pleomorphism, a sinusoidal growth pattern, and Ki-1 epitope reactivity. This type of lymphoma is often mistaken for metastatic carcinoma, melanoma, or malignant histiocytosis. Although persons with acquired immunodeficiency syndrome frequently have non-Hodgkin's lymphoma at extranodal sites, the oral cavity and mandible, in particular, are unusual locations. We report two cases of anaplastic large-cell Ki-1 lymphoma that occurred in persons with the human immunodeficiency virus and with initial presentation as soft tissue masses of the posterior mandible. Immunocytochemical studies were positive for Ki-1 (CD30) in both cases. in situ hybridization for Epstein-Barr virus-deoxyribonucleic acid was positive with tumor cells in both cases. flow cytometry on paraffin, formalin-fixed tissue revealed tetraploidy and high proliferative fractions that are characteristic of high-grade lymphomas. Intraoral presentation of rapidly enlarging, soft tissue masses may represent a high-grade non-Hodgkin's lymphoma in persons with the human immunodeficiency virus. Although rare, anaplastic large-cell Ki-1 lymphoma should be considered and requires immunocytochemical study to eliminate the possibility of other malignant conditions associated with the acquired immunodeficiency syndrome.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |