1/4. Verruciform xanthoma of the ear with coexisting epidermal dysplasia.We report a case of verruciform xanthoma of the ear in a 79-year-old man. The case is unique in that it contained an area of solar keratosis. in situ hybridization using biotin-labeled probe cocktails for human papillomavirus types 6/11, 16/18, and 31/33/35 yielded negative results.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/4. Seborrheic keratoses from the genital region may contain human papillomavirus dna.Sections from paraffin-embedded tissues of lesions interpreted as seborrheic keratoses localized to the pubic, genital, and crural regions were assayed for the presence of human papillomavirus types 6, 11, 16, 18, and 33 using dna amplification followed by specific hybridization. Lesions with the histologic characteristics of condyloma were excluded from the study. Human papillomavirus dna sequences were found in 24 (42%) of 57 seborrheic keratosis-like lesions from the genital region. No human papillomavirus dna was detected in 27 control specimens that represented a variety of other processes occurring in this area. We conclude that human papillomavirus infection cannot be excluded in genital seborrheic keratoses.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
3/4. Detection of human papillomavirus dna in an ameloblastoma using the in situ hybridization technique.HPV type 18 dna was identified in an intrabony ameloblastoma using radiolabelled in situ hybridization. The viral dna was found in a verrucous lesion in a cystic area of the tumor. The absence of HPV dna in other epithelial areas of the ameloblastoma is suggestive of a secondary infection. HPV is not considered to be an etiological factor in the pathogenesis of this ameloblastoma.- - - - - - - - - - ranking = 5keywords = hybridization (Clic here for more details about this article) |
4/4. Detection of new human papillomavirus sequences in skin lesions of a renal transplant recipient and characterization of one complete genome related to epidermodysplasia verruciformis-associated types.Human papillomavirus (HPV) dna, originally isolated from patients suffering from the skin disease epidermodysplasia verruciformis (EV), and a growing number of related sequences have recently been detected in a high percentage of benign and malignant skin lesions of both immunosuppressed and immunocompetent people. HPV L1 dna fragments (374-389 bp long) from a solar keratosis and a squamous cell carcinoma (SCC) of a renal transplant recipient were amplified, cloned and sequenced. In 54 clones, six different HPV sequences were identified. One of these six corresponded to the known type HPV-8 and two (RTRX3 and RTRX7) have been described previously in cutaneous lesions of immunosuppressed patients. The remaining three sequences were different from all known HPV types: an HPV-9-related sequence (77.4% identity), an RTRX2-related sequence (82.6% identity), and an HPV-22-related sequence (83.7% identity). These three sequences, representing putatively new HPV types, were named RTRX8, RTRX9 and RTRX10, respectively. RTRX7 was found in the majority of clones from both lesions. The complete genome of RTRX7 (7731 bp) was cloned as six overlapping subgenomic fragments, generated by nested PCR with dna extracts from the SCC. RTRX7 showed a genome organization typical of HPVs associated with EV. The L1 dna sequence differed by 15% from the corresponding region of its closest known relative, HPV-12; thus, RTRX7 can be regarded as a new HPV type. RTRX7 dna could not be detected by Southern blot hybridization with the homologous probe, indicating that the dna concentration was below one copy per 10 cells in the investigated SCC.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |