1/3. Fatal neonatal pneumonia caused by adenovirus type 35. Report of one case and review of the literature.A 3680-g term male neonate developed bilateral bronchopneumonia at 9 days of age. The labor, delivery, and immediate postnatal period had been unremarkable. Despite standard antibiotic therapy, the patient progressed to respiratory failure and died 4 days later. Adenovirus particles were found in oropharyngeal secretions 1 day prior to death. autopsy revealed an extensive necrotizing bronchiolitis and alveolitis with frequent "smudge cells." Adenovirus was identified by culture, electron microscopy, and in situ dna hybridization. The adenovirus was serotyped as type 35, which, to our knowledge, has not been previously described in neonatal adenovirus pneumonia.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/3. Adenovirus encephalitis and widespread ependymitis in a child with AIDS.A 4-year-old child with AIDS developed encephalitis 2 months prior to death. Adenovirus was cultured from the cerebrospinal fluid. At autopsy, sections of the brain revealed complete sloughing of the intracranial ependyma, with marked gliosis and edema of the periventricular white matter. Cells with large, dark, smudgy intranuclear inclusions, consistent with adenovirus inclusions, were also seen in subependymal locations. in situ hybridization studies confirmed the presence of adenovirus dna in these cells. This represents a case of morphologically proven adenovirus encephalitis in a pediatric AIDS patient.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
3/3. Neonatal adenovirus infection: a case report with in situ hybridization confirmation of ascending intrauterine infection.Adenovirus infection is a rare, but serious infection, during the neonatal period. The actual model of infection at birth is currently unknown, however, several mechanisms have been proposed. We describe a case of fatal neonatal adenovirus pneumonia in a 25-wk gestational age infant. Adenovirus was confirmed by electron microscopy and by in situ hybridization. The maternal cervical/endocervical smear taken prior to the delivery contained epithelial cells with changes suggestive of adenovirus, which was confirmed by in situ hybridization on the smear. These findings suggest that ascending viral infection is a factor in the pathogenesis of neonatal adenovirus infection. The identification and reporting of adenovirus may be important during pregnancy in order to avoid delay in delivery of the fetus once membranes have ruptured.- - - - - - - - - - ranking = 6keywords = hybridization (Clic here for more details about this article) |