1/2. epidermodysplasia verruciformis-associated papillomavirus infection complicating human immunodeficiency virus disease.Three males infected with the human immunodeficiency virus (hiv) were noted to have extensive flat warts of the face and/or body. In two there were also pityriasis versicolor-like lesions. Biopsies showed foamy, basophilic, distended cytoplasm in granular layer keratinocytes, characteristic of the human papillomavirus types seen in epidermodysplasia verruciformis. dna hybridization techniques demonstrated the presence of HPV-type 8 in one patient and HPV 5 and 8 in another. patients with immune suppression due to hiv infection may demonstrate the clinical features of epidermodysplasia verruciformis with the same potentially oncogenic HPV types.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/2. Two different human papillomavirus (HPV) types associated with oral mucosal lesions in an hiv-seropositive man.Different types of Human papillomaviruses (HPV) are associated with a variety of oral lesions. So far, HPV types 1, 2, 4, 6, 7, 11, 13, 16, 18, 32 and 57 have been identified in oral lesions. immunosuppression predisposes oral mucosa to clinical manifestation of different virus infections including HPV. We describe here a 30-year-old hiv-positive and immunosuppressed man, who had suffered from oral lesions for a few months. On clinical examination, a nodular elevation was detected on the lower lip, and white keratotic areas were present on buccal mucosa bilaterally. A biopsy from the lip revealed the presence of acanthosis with a prominent granular cell layer as well as hyperparakeratosis. A biopsy from the buccal lesion showed a comparatively much flatter lesion with merely basal cell hyperplasia associated with hyperparakeratosis. Koilocytosis was a characteristic feature in both biopsies. In Southern blot hybridization, both lesions hybridized with a probe cocktail comprising HPV 6, 11, 16, 18, 31 and 33 dna under low stringency. Under high stringency, the lip lesion proved to contain HPV 7 dna, which also confirmed by in situ hybridization. The buccal lesion was weakly positive by Southern blot with HPV 11 and 13 probes hybridized under stringent conditions, but the restriction patterns with Pst I and Bam HI did not fit with those of any of the 57 HPV types known so far. In situ hybridizations with HPV 11 and HPV 13 probes were negative. Cloning of this 'new' HPV type is currently under way.- - - - - - - - - - ranking = 3keywords = hybridization (Clic here for more details about this article) |