Récepteur Purinergique P1
A class of cell surface receptors that prefers ADENOSINE to other endogenous purines
. Purinergic P1 receptors are widespread in the body including the cardiovascular, respiratory, immune, and nervous systems. There are at least two pharmacologically distinguishable types (A1 and A2, or
Ri and Ra). The methylxanthines, e.g., CAFFEINE, bind to these receptors, but also have other unrelated effects.
Récepteur Purinergique P1
Récepteur Purinergique P1
Récepteur Purinergique P1
Récepteur Purinergique P1
Récepteur Purinergique (methyladenine receptors; purine receptor;
- Receptors, Purinergic P2X7
- Receptors, Purinergic P2X
- Receptors, Purinergic P2Y2
- Receptors, Purinergic P2X3
- Récepteur Purinergique: Cell surface proteins that bind PURINES with high affinity and trigger intracellular changes which influence the behavior of cells. The best characterized classes of purinergic receptors in mammals are the P1 receptors, which prefer ADENOSINE, and the P2 receptors, which prefer ATP or ADP.
- Receptors, Purinergic P2Y1
- Purinergic P2 Receptor Antagonists
- Purinergic Agonists
- Receptors, Purinergic P2X2
- Purinergic P2 Receptor Agonists
- Récepteur Purinergique P2: A class of cell surface receptors for purines that prefer ATP or ADP over adenosine. P2 purinergic receptors are widespread in the periphery and in the central and peripheral nervous system. Subtypes have been proposed, usually designated P2 x, y, z, and t. P2x receptors may mediate fast synaptic transmission by ATP. The ADP-preferring P2t receptors in platelets stimulate aggregation.
- Purinergic Antagonists
- Receptors, Purinergic P2X4
- Receptors, Purinergic P2X5
- Receptors, Purinergic P2Y
- Humains: Membres de l'espèce Homo sapiens.
- Purinergic P2X Receptor Antagonists
- Purinergic P2X Receptor Agonists
- Receptors, Purinergic P2X1
- Receptors, Purinergic P2Y12
- Purinergic P2Y Receptor Agonists
- Animaux: Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain Eukarya.
- Purinergic P1 Receptor Agonists
- Purinergic P1 Receptor Antagonists
- Récepteur Purinergique P1: A class of cell surface receptors that prefers ADENOSINE to other endogenous purines. Purinergic P1 receptors are widespread in the body including the cardiovascular, respiratory, immune, and nervous systems. There are at least two pharmacologically distinguishable types (A1 and A2, or Ri and Ra). The methylxanthines, e.g., CAFFEINE, bind to these receptors, but also have other unrelated effects.
- Rats: The common name for the genus Rattus.
- Purinergic Agents
- Adénosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
- Cellules Cancéreuses En Culture: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
- Dinucléoside Phosphates: A group of compounds which consist of a nucleotide molecule to which an additional nucleoside is attached through the phosphate molecule(s). The nucleotide can contain any number of phosphates.
- Lignée Cellulaire: Established cell cultures that have the potential to propagate indefinitely.
- Nucleotidases: A class of enzymes that catalyze the conversion of a nucleotide and water to a nucleoside and orthophosphate. EC 3.1.3.-.
- Transduction Signal: The intercellular or intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
- Apyrase: A calcium-activated enzyme that catalyzes the hydrolysis of ATP to yield AMP and orthophosphate. It can also act on ADP and other nucleoside triphosphates and diphosphates. EC 220.127.116.11.
- Signalisation Par Calcium: Signal transduction mechanisms whereby calcium mobilization (from outside the cell or from intracellular storage pools) to the cytoplasm is triggered by external stimuli. Calcium signals are often seen to propagate as waves, oscillations, spikes or puffs. The calcium acts as an intracellular messenger by activating calcium-responsive proteins.
- Microscopie : Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
- Adp: Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
- Transmission Nerveuse: The communication from a neuron to a target (neuron, muscle, or secretory cell) across a synapse. In chemical synaptic transmission, the presynaptic neuron releases a neurotransmitter that diffuses across the synaptic cleft and binds to specific synaptic receptors. These activated receptors modulate ion channels and/or second-messenger systems to influence the postsynaptic cell. Electrical transmission is less common in the nervous system, and, as in other tissues, is mediated by gap junctions.
- Nucléotide Adénylique
- Purinergic P2Y Receptor Antagonists
- Agents Neuromédiateurs: Substances utilisées pour leurs actions pharmacologiques sur les systèmes de neurotransmetteurs. Les agents neurotransmetteurs incluent des agonistes, antagonistes, inhibiteurs, dépleteurs, précurseurs, et les modulateurs du fonctionnement du récepteur.
- Nucléotide: http://www.orpha.net/gor/cgi-bin//OGmain.php?Lng=FR&Typ=Ident&Val=269.0
- Fura-2: A fluorescent calcium chelating agent which is used to study intracellular calcium in many tissues. The fluorescent and chelating properties of Fura-2 aid in the quantitation of endothelial cell injury, in monitoring ATP-dependent calcium uptake by membrane vesicles, and in the determination of the relationship between cytoplasmic free calcium and oxidase activation in rat neutrophils.
- Techniques Patch-Clamp: An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used.
- Transport Ionique: The movement of ions across energy-transducing cell membranes. Transport can be active, passive or facilitated. Ions may travel by themselves (uniport), or as a group of two or more ions in the same (symport) or opposite (antiport) directions.
- Communication Paracrine: Signalisation cellulaire par laquelle un facteur sécrété par une cellule affecte d'autres cellules dans son voisinage. Ce terme est souvent employé pour désigner l'action des hormones sur les cellules environnantes.
- Perméabilité Membrane Cellulaire: A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
- Souris Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
- Modèle Biologique: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
- HEK293 Cells
- Réaction Polymérisation En Chaîne Par Transcriptase Inverse: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
- Lignée Cellulaire Tumorale: A cell line derived from cultured tumor cells.
- Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
- Relation Dose-Effet Médicaments: The relationship between the dose of an administered drug and the response of the organism to the drug.
- Rat Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
- Potentiels De Membrane: The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES' membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
- Cochons D'Inde: A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
- Souris De Lignée C57Bl
- Adulte D'
- Adulte: A person having attained full growth or maturity. Adults are of 19 through 44 years of age.
- Ligands: A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
- Stimulation : Use of electric potential or currents to elicit biological responses.
- Données Séquence Moléculaire: Descriptions de séquences d'acides aminés, de glucides, ou de nucléotides qui sont publiées dans la littérature et/ou sont brevetées et stockées dans des banques de données telles que GenBank, le laboratoire moléculaire européen de biologie (EMBL), la fondation biomédicale nationale de recherches (NBRF), ou d'autres dépositaires de séquences.
- Far-Western Blot: A method that is derived from western blotting (BLOTTING, WESTERN) and is used to detect protein-protein interactions. The blotted proteins are probed with a non-antibody protein which can then be tagged with a labeled antibody.
- Purines: A series of heterocyclic compounds that are variously substituted in nature and are known also as purine bases. They include ADENINE and GUANINE, constituents of nucleic acids, as well as many alkaloids such as CAFFEINE and THEOPHYLLINE. Uric acid is the metabolic end product of purine metabolism.
- Rat Wistar: A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
- Hydrolyse: The process of cleaving a chemical compound by the addition of a molecule of water.
- Immunohistochimie: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
- Cinétique: L'étude des temps de réaction dans les systèmes chimiques ou physiques.
- Régulation Expression Génique: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action at the level of transcription or translation. These processes include gene activation and genetic induction.
- Cellules Cho: CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
- Activation Enzymatique: Conversion d'une forme inactive d'une enzyme en une forme ayant une activité métabolique. Inclut : 1, l'activation par des ions (activateurs) ; 2, l'activation par co-facteurs (coenzymes) ; et 3, la conversion d'un précurseur enzymatique (proenzyme ou zymogène) en enzyme active.
- CISMeF - Catalogue et Index des Sites Médicaux Francophones.
- OMS - Organisation Mondiale de la Santé.