A transmembrane domain containing ephrin that binds with high affinity to EPHB1 RECEPTOR; EPHB3 RECEPTOR; and EPHB4 RECEPTOR. Expression of ephrin-b2 occurs in a variety of adult tissues. During embryogenesis, high levels of ephrin-b2 is seen in the prosencephalon; rhombencephalon; developing somites; LIMB BUD; and bronchial arches.