1/4. Haemorrhage in neonatal hepatic necrosis due to herpes infection.A case of early neonatal severe bleeding and persistent hypoglycemia with a fatal outcome is reported. The autopsy examination revealed the features of neonatal hepatic necrosis. Further study by the electron microscopy indicated the presence of herpes type particles in the nucleus and cytoplasm of the remaining liver cells. Serological study of the maternal blood, post partum, revelaed positive reaction to herpes simplex virus type 2 at low titer. It is believed that intrauterine herpes infection was responsible for the severe hepatic damage manifesting in complex clinical findings.- - - - - - - - - - ranking = 1keywords = nucleus (Clic here for more details about this article) |
2/4. herpes simplex-hepatitus use of immunoperoxidase to demonstrate the viral antigen in hepatocytes.In this paper we describe 2 patients with herpes simplex hepatitis. Submassive liver necrosis occurred in both patients, one of whom survived. Notable in the clinical course of both patients was the almost simultaneous occurrence of three events-fever, marked elevation of serum transaminases, and leukopenia. Using an immunoperoxidase staining technique we demonstrated herpes simplex viral antigen in the nucleus, cytoplasm, and cell membrane of affected hepatocytes.- - - - - - - - - - ranking = 1keywords = nucleus (Clic here for more details about this article) |
3/4. Ultrastructural changes in human hepatocytes in acute non-A, non-B hepatitis.Ultrastructural findings in the liver of a 52-year-old man with acute non-A, non-B (NANB) post-transfusion hepatitis are described. Apart from non-specific alterations also known to occur in hepatocytes in hepatitis a and B--such as proliferation of membranes of smooth endoplasmic reticulum, formation of membrane-bound cytoplasmic vacuoles containing electron-dense material, and accumulation of distorted peroxisomes--unique cytoplasmic changes were observed that have not previously been described in man. A few hepatocytes contained in their cytoplasm tightly packed, bent, parallel structures and small clusters of virus-sized particles. No virus-like material was found in the nucleus of liver cells or in Kupffer and endothelial cells. Closely similar structures have been reported earlier in the acute-phase hepatocytic cytoplasm of chimpanzees with NANB hepatitis. These alterations may represent an ultrastructural hallmark of acute human NANB hepatitis.- - - - - - - - - - ranking = 1keywords = nucleus (Clic here for more details about this article) |
4/4. Non-A, non-B hepatitis: a case report.Ultrastructural changes in a liver biopsy obtained for diagnostic purposes from a patient with suspected non-A, non-B hepatitis were consistent with hepatitis and included dilatation and disaggregation of the endoplasmic reticulum, hepatocyte disarray, and variations in mitochondrial size. In addition, some foci of hepatocytes showed atypical cytoplasmic changes including intranuclear cytoplasmic pseudo-inclusions, consisting basically of invaginations of the cytoplasmic mass into the nucleus, glycogen deposits in the same nuclei, and frequently accumulations of fat droplets. The mitochondria were generally pleomorphic, and occasionally the mitochondrial cristae were arranged longitudinally. dilatation, hypertrophy, and distortion of the Golgi complexes were found in affected hepatocytes. Similar ultrastructural changes have previously been reported in human hepatocellular carcinoma as well as in carcinomas of other tissues.- - - - - - - - - - ranking = 1keywords = nucleus (Clic here for more details about this article) |