1/3. A patient with Crimean-congo hemorrhagic fever serologically diagnosed by recombinant nucleoprotein-based antibody detection systems.We treated a male patient with Crimean-congo hemorrhagic fever (CCHF). The diagnosis of CCHF was confirmed by reverse transcription-PCR and recombinant nucleoprotein (rNP)-based immunoglobulin g (IgG) and IgM capture enzyme-linked immunosorbent assays of serially collected serum samples. The patient was treated with intravenous ribavirin and recovered with no consequences. The study indicates that rNP-based CCHF virus antibody detection systems are useful for confirming CCHF virus infections. This case also suggests that intravenous ribavirin therapy may be promising for the treatment of CCHF patients.- - - - - - - - - - ranking = 1keywords = sample (Clic here for more details about this article) |
2/3. Genotoxic effect of ribavirin in patients with Crimean-congo hemorrhagic fever.In this study, we investigated the in vivo genotoxicity of ribavirin in humans, studying 3 patients with Crimean-congo hemorrhagic fever who were treated with high-dose ribavirin. In order to evaluate genotoxicity, both the micronucleus (MN) test and the sister chromatid exchange (SCE) test were used. In all patients, blood samples were taken during and after therapy. Whole blood cultures were performed for 72 h and the MN assay and SCE test were then carried out to demonstrate the genotoxicity. In all patients, both SCE and MN amounts were found to be higher in the samples which were taken during therapy than in those at 1 month after therapy. The results of our study reveal that ribavirin has a reversible in vivo genotoxic effect on humans.- - - - - - - - - - ranking = 2keywords = sample (Clic here for more details about this article) |
3/3. Investigation of tick-borne viruses as pathogens of humans in south africa and evidence of Dugbe virus infection in a patient with prolonged thrombocytopenia.In the course of investigating suspected cases of viral haemorrhagic fever in south africa patients were encountered who had been bitten by ticks, but who lacked evidence of infection with Crimean-congo haemorrhagic fever (CCHF) virus or non-viral tick-borne agents. cattle sera were tested by enzyme-linked immunoassay to determine whether tick-borne viruses other than CCHF occur in the country. The prevalence of antibody in cattle sera was 905/2116 (42.8%) for CCHF virus, 70/1358 (5.2%) for Dugbe, 21/1358 (1.5%) for louping ill, 6/450 (1.3%) for West Nile, 7/1358 (0.5%) for nairobi sheep disease, 3/625 (0.5%) for Kadam and 2/450 (0.4%) for Chenuda. No reactions were recorded with Hazara, Bahig, Bhanja, Thogoto and Dhori viruses. The CCHF findings confirmed previous observations that the virus is widely prevalent within the distribution range of ticks of the genus Hyalomma, while antibody activity to Dugbe antigen was detected only within the distribution range of the tick Amblyomma hebraeum. Cross-reactivity for the nairoviruses, Hazara, nairobi sheep disease and Dugbe, was detected in serum samples from 3/72 human patients with confirmed CCHF infection, and serum from 1/162 other patients reacted monospecifically with Dugbe antigen. The latter patient suffered from febrile illness with prolonged thrombocytopenia.- - - - - - - - - - ranking = 1keywords = sample (Clic here for more details about this article) |