1/5. Primary Ewing's sarcoma family of tumors arising from the broad ligament.We report a case of a primary Ewing's sarcoma family of tumors (ESFT) that was an incidental finding in the broad ligament of a 53-year-old woman. ESFT now includes tumors previously described as Askin tumor, neuroepithelioma, extraskeletal Ewing's sarcoma, and peripheral primitive neuroectodermal tumor. This is because of the discovery that all of the above tumors contain a specific gene rearrangement involving chromosome 22q12. On microscopic examination, the tumor was composed of small, round cells with mild nuclear pleomorphism and scant eosinophilic cytoplasm. There were large areas of tumor necrosis and numerous mitoses. Immunohistochemically, there was strong membrane staining for CD99 and weak focal staining for CD56 and neuron specific enolase. fluorescence in situ hybridization revealed a separation of the breakapart probe on chromosome 22q12 consistent with the presence of a gene rearrangement, supporting the diagnosis of ESFT. We believe the importance of recognizing the existence of a primary ESFT in the broad ligament as some primary tumors of the gynecological tract and of other systems may resemble it histologically. To the best of our knowledge, our case is the second case of extraskeletal Ewings sarcoma arising from the broad ligament but the first case that is confirmed by strong CD99 positivity and supported by fluorescence in situ hybridization.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/5. Human papillomavirus types 6 and 16 in multifocal intraepithelial neoplasias of the female lower genital tract.Five women with multifocal intraepithelial neoplasia of the lower genital tract were investigated for the presence of human papillomavirus (HPV) infection by the method of dna-dna hybridization which detects the viral dna. The dna sequences of HPV types 6 and 16 were detected in each of the five patients and in each of the areas biopsied: cervix, vagina and vulva. dna sequences of both viral types were also found in vulval intraepithelial neoplasia grades I-III and in cervical intraepithelial neoplasia grades I and III. The detection of HPV DNAs in multifocal lesions suggests a possible common aetiology for the lower genital tract intraepithelial neoplasias.- - - - - - - - - - ranking = 0.5keywords = hybridization (Clic here for more details about this article) |
3/5. Genital tract papillomavirus infection in children.Genital tract papillomas in five children were examined for the presence of human papillomavirus (HPV) dna by molecular hybridization. Papillomavirus dna was detected in each sample and was identified as HPV-6 (three cases), HPV-6 or HPV-11 (one case), or HPV-16 (one case). These viruses are the same as are responsible for genital papillomas (condylomata) of adults. The transmission of adult genital tract viruses to children occurs primarily by a venereal route but may occur by a nonvenereal route.- - - - - - - - - - ranking = 0.5keywords = hybridization (Clic here for more details about this article) |
4/5. female genital tumors associated with human papillomavirus infection, and the concept of genital neoplasm-papilloma syndrome (GENPS).With the recent development of new analytic methods, notably the dna hybridization technique, many benign and malignant gynecologic tumors including carcinoma in situ, verrucous carcinoma and some invasive carcinomas of the vulva, the vagina and the cervix are found to be associated with human papillomavirus infection. Benign warts and multiple neoplasms frequently appear synchronously or metachronously in a single patient, and thus present as the genital neoplasm-papilloma syndrome (GENPS). Various methods of human papillomavirus identification and a spectrum of benign and malignant female genital tumors proven to contain human papillomavirus are reviewed and summarized.- - - - - - - - - - ranking = 0.5keywords = hybridization (Clic here for more details about this article) |
5/5. Human papillomavirus, type 16, dna in multicentric anogenital neoplasia associated with idiopathic panmyelopathy. A case report.A 27-year-old woman suffering from panmyelopathy for six years presented with a cervical low grade squamous intraepithelial lesion (SIL), vulvar high grade SIL and perianal squamous cell carcinoma with an inguinal metastasis. Southern blot hybridization with 32P-labeled human papillomavirus (HPV) dna revealed HPV 16 dna in varying copy numbers in material from the four locations. HPV 16 genomes persisting after surgery on the perianal tumor area were no longer detectable after betatron radiotherapy.- - - - - - - - - - ranking = 0.5keywords = hybridization (Clic here for more details about this article) |