1/17. The presence of HPV types 6/11, 16/18, 31/33/51 in Bowenoid papulosis demonstrated by dna in situ hybridization.Bowenoid papulosis (BP) is an unusual dermatosis with variable clinical presentation: multiple, generally small, round, papules, isolated or confluent, with smooth or papillomatous surface, sometimes with desquamation. The colour is variable from rose, reddish-purple to brown. These papules are localized mostly on the genital mucosa or perigenital skin of young adults. The aetiopathogenesis of the disorder is not well defined, but it may be linked to human papillomavirus (HPV) infection. We report a case of BP with some particular aspects: (1) misdiagnosis of the disease for 2 years; (2) contemporaneous presence of different types of HPV.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/17. Human papillomavirus type 58 in bowen's disease of the elbow.Human papillomavirus (HPV) can be detected in skin lesions of bowen's disease, particularly on the fingers, and its genotype is associated with mucosal/genital types of HPV. We report herein an 85-year-old woman who had HPV-associated bowen's disease on her elbow. HPV-58 dna was detected in the lesion by polymerase chain reaction with restriction fragment length polymorphism and by Southern blot hybridization. in situ hybridization revealed numerous hybrid cells in the nuclei of the upper epidermis and stratum corneum of bowen's disease. A high-risk type of mucosal HPV-58 dna is associated with bowen's disease in this case, suggesting that HPV-related bowen's disease is not always restricted to genital or finger lesions.- - - - - - - - - - ranking = 0.5keywords = hybridization (Clic here for more details about this article) |
3/17. Human papillomavirus associated bowen's disease of the foot: unique clinical features mimicking a common wart.We describe a case of bowen's disease that developed on the left dorsal foot of a 56-year-old female patient and that mimicked the clinical features of a common wart. PCR-based analysis indicated that the lesion contained HPV-16 dna and HPV-16 positive cells were found in the upper layers of the epidermal lesion using in situ hybridization with an HPV-16 probe.- - - - - - - - - - ranking = 0.25keywords = hybridization (Clic here for more details about this article) |
4/17. A cardiac allograft recipient with bowen's disease on a finger and concurrent perianal bowenoid papulosis.We report a patient who developed bowen's disease of the finger and bowenoid papulosis of the perianal area after cardiac transplantation. Human papillomavirus (HPV) type 16 only, not any skin-related or epidermodysplasia verruciformis-related types, was detected in both lesions by in situ hybridization and polymerase chain reaction. The same virus type was identified in both the tumor of the finger and the perianal area, which suggests contact transmission. HPV 16 has often been associated with malignant changes and may be at least one source of the malignancies that are more common in immunosuppressed patients. The presence of a potentially oncogenic type of the HPV in an immunosuppressed patient highlights the importance of regular follow-up of such patients.- - - - - - - - - - ranking = 0.25keywords = hybridization (Clic here for more details about this article) |
5/17. Triple cancers in the urogenital area of a patient with aplastic anemia.Three epithelial neoplastic lesions, perineal Bowenoid papulosis, uterine cervical carcinoma, and bladder transitional cell carcinoma, which occurred in a mildly immunosuppressed patient who had aplastic anemia were studied for human papillomavirus (HPV) infection. In the Bowenoid papulosis, HPV type 16 dna was identified by polymerase chain reaction (PCR) and by in situ hybridization (ISH). In contrast, in the uterine cervical carcinoma, HPV 16 was not detected, although possibly another unidentified type of HPV in the lesion was suggested by the ISH findings. In the bladder transitional cell carcinoma, neither papillomavirus genus-specific (PGS) antigen nor HPV dna was found.- - - - - - - - - - ranking = 0.25keywords = hybridization (Clic here for more details about this article) |
6/17. Bowen's carcinoma of the scrotal skin associated with human papillomavirus type 82.We have previously cloned human papillomavirus type 82 (HPV-82) from a vaginal intraepithelial neoplasia, but it is not known whether HPV-82 can induce a cutaneous lesion. A large erosive nodule developed on the scrotum of a 50-year-old Japanese patient. Histopathologically, the lesion was composed of two distinct parts; one part showing changes characteristic of bowen's disease in the epidermis, and the other showing elongated rete ridges and proliferation of atypical basaloid cells in the dermis. These parts were partially connected, giving the diagnosis of Bowen's carcinoma. Immunohistochemically, HPV capsid antigen was detected only in the nuclei of a few cells on the upper part of the epidermis. HPV-82 was identified in the lesion by blot hybridization and viral dna was demonstrated in the lesion by in situ hybridization. HPV-82 has tropism for both the skin and the genital regions.- - - - - - - - - - ranking = 0.5keywords = hybridization (Clic here for more details about this article) |
7/17. Simultaneous human papillomavirus 6 (HPV 6) -positive condyloma acuminatum, HPV 31-positive bowen's disease, and non HPV-associated extramammary Paget's disease coexisting within an area presenting clinically as condyloma acuminatum.An 83-year-old Japanese man presented with multiple verrucous papules clustering on a plaque located on the frontal aspect of the scrotum. Histologically, there were three distinct epithelial changes compatible with condyloma acuminatum, bowen's disease, and extramammary Paget's disease (EMPD). By in situ hybridization, the zone of condyloma acuminatum was positive for HPV 6 and well demarcated from HPV 31-positive bowen's disease. EMPD was negative for targeted HPV 6/11/16/18/31/33 probes. Immunohistochemically, Paget's cells expressing cytokeratin 7 were distributed as scattered single cells or clusters mainly in the lower part of the HPV 6/31-positive epithelium. To the best of our knowledge, this is the first reported case of the occurrence of condyloma acuminatum, bowen's disease, and EMPD within the same lesion.- - - - - - - - - - ranking = 0.25keywords = hybridization (Clic here for more details about this article) |
8/17. epidermodysplasia verruciformis. A case associated with primary lymphatic dysplasia, depressed cell-mediated immunity, and bowen's disease containing human papillomavirus 16 dna.epidermodysplasia verruciformis is a rare, often hereditary disease characterized by a generalized cutaneous infection with human papillomavirus (HPV), depressed cell-mediated immunity, and a propensity for transformation of the warty lesions to squamous cell carcinoma on primarily sun-exposed areas of the skin. A 37-year-old man with congenital lymphatic dysplasia and a history of squamous cell carcinoma of the groin and foot was observed by us to have edema of all four extremities, numerous flat warts, and pityriasis versicolor-like papules over the trunk and arms. Condylomatous lesions were noted in the groin and a periungual verrucous nodule on the thumb. Biopsies showed the trunk and arm lesions to be verrucae and the thumb lesion to be bowen's disease. Results of molecular hybridization studies from four lesions of the arms showed the presence of only HPV 3 dna; HPV 16-related dna was detected in the intraepidermal carcinoma on the thumb. Immunologic evaluation revealed anergy to routine skin testing, depressed mitogen-stimulated lymphocyte transformation, decreased B-lymphocyte count, and a severe reversal of the T-lymphocyte helper:suppressor ratio.- - - - - - - - - - ranking = 0.25keywords = hybridization (Clic here for more details about this article) |
9/17. bowen's disease of the feet. Presence of human papillomavirus 16 dna in tumor tissue.A 36-year-old black man with bilateral squamous cell carcinoma in situ of the feet is described. The dna hybridization analysis performed on the tumor tissue demonstrated the presence of human papillomavirus 16 dna. human papillomavirus 16 has been detected repeatedly in genital carcinomas, and evidence is mounting that it may play an important role in the development of malignancy.- - - - - - - - - - ranking = 0.25keywords = hybridization (Clic here for more details about this article) |
10/17. Multifocal invasive carcinoma of the vulva in a 25-year-old woman with Bowenoid papulosis.This case report documents malignant progression associated with wart virus infection of the vulva in a 25-year-old female. The initial condition of Bowenoid papulosis and carcinoma in situ of the vulva was diagnosed on colposcopic biopsies performed to investigate chronic pruritus and superficial dyspareunia. This condition failed to resolve with local ablative therapy and progressed over a period of 8 months to multifocal invasive carcinoma of the vulva requiring radical surgery. Deoxyribonucleic acid hybridization studies on the operative specimen revealed the presence of human papilloma virus type 16. The role of human papilloma virus in the aetiology of Bowenoid papulosis and neoplasia of the vulva is discussed.- - - - - - - - - - ranking = 0.25keywords = hybridization (Clic here for more details about this article) |
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