1/5. Characteristic intracytoplasmic material in a perianal wart that contains human papillomavirus type 56 and 16 dna.We report the case of a perianal wart in which human papillomavirus type 56 and 16 DNAs were detected, arising in a 29-year-old healthy Japanese female. Clinically, moist erythema and small flat pinkish papules appeared around the anus and worsened over a period of 6 months. Histopathologically, no signs of dysplasia or atypia were apparent. An area of intracytoplasmic material, which was homogeneous and eosinophilic, was contained in each epidermal cell. The wart showed HPV-56 and HPV-16 DNAs by PCR and reverse-phase dot blot hybridization. Additional Southern blot analysis showed the amount of HPV-56 dna to be more than 10 times that of HPV-16 dna. This is the first case, to our knowledge, of a perianal wart with intracytoplasmic material, which contains HPV DNAs causing mucous lesions.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
2/5. herpes simplex virus type-2 ulcers resistant to acyclovir in an AIDS patient--successful treatment with foscarnet.The case of a 25-year-old homosexual man with large inguinal and perianal ulcers is reported. He had been pretreated extensively with acyclovir. herpes simplex virus (HSV) was identified in the ulcer tissue by in-situ hybridization. No clinical improvement with acyclovir but a prompt response to foscarnet was noted. In a relapse after 11 weeks an acyclovir-resistant HSV type 2 was isolated. Again, a prompt response to foscarnet was noted. The case is presented in detail, and the clinical impact of resistance of HSV to acyclovir is discussed.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
3/5. Concurrent HPV-16 infection of the nipple and perianal area in an hiv-1 patient. Military Medical Consortium for the Advancement of Retroviral research (MMCARR)BACKGROUND. In patients with hiv-1 disease there has been an increasing association with human papilloma virus (HPV) infections in multiple locations as well as an increase in associated tumors. In addition, there has been increased recovery of HPV in individuals with decreasing T4 cell counts. CASE REPORT. Recently we have seen an hiv-1 patient with a cutaneous lesion on the nipple, as well as multiple perianal lesions in which HPV-16 was demonstrated by in-situ hybridization. Although these lesions contained the same subtype of HPV virus, they had very different clinical and histopathologic morphologies, and this represents the first reported association of HPV-16 in a nipple lesion. DISCUSSION. Our patient illustrates that in hiv-1 disease, HPV infections may present in more diffuse and atypical locations. In addition, the diffuse staining with the in-situ probe for HIV-16 within the lesions, tends to support the findings of others, that viral recovery increases with the immune suppression induced by hiv-1.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
4/5. Condyloma acuminatum with superficial spirochetosis simulating condyloma latum.We report a case of a 12-year-old boy with a large perianal condyloma. The lesion was found to contain both spirochetes (Warthin-Starry stain) and human papillomavirus types 6 or 11 (in situ hybridization). Because of persistent negative serologies for syphilis and lack of other clinical stigmata of syphilis, the lesion was diagnosed as condyloma acuminatum with secondary involvement by spirochetes of probable intestinal origin. The case demonstrates the potential histologic similarities between condyloma latum and condyloma acuminatum, as well as the pitfalls of using the Warthin-Starry stain to establish the diagnosis of secondary syphilis.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |
5/5. Perianal cytomegalovirus ulcer in an HIV-infected patient.A 51-year-old man with acquired immunodeficiency syndrome presented with a perianal ulcer. Histologically there were numerous cytomegalic inclusions within endothelial cells and stomal fibroblasts. cytomegalovirus within infected cells was demonstrated by immunohistochemistry and in situ hybridization. No cytopathic typical of herpes simplex were detected. The ulcer resolved completely after a treatment with ganciclovir.- - - - - - - - - - ranking = 1keywords = hybridization (Clic here for more details about this article) |